BMRB Entry 52248

Title:
1H and 15N Chemical Shifts of PICK1 without alpha7nAChR ICD+TMD
Deposition date:
2023-12-20
Original release date:
2024-05-02
Authors:
Bondarenko, Vasyl; Chen, Qiang; Tang, Pei
Citation:

Citation: Bondarenko, Vasyl; Chen, Qiang; Tillman, Tommy; Xu, Yan; Tang, Pei. "Unconventional PDZ Recognition Revealed in a7 nAChR-PICK1 Complexes"  ACS Chem. Neurosci. 15, 2070-2079 (2024).
PubMed: 38691676

Assembly members:

Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTBSG1-PICK104

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts81
1H chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PICK11

Entities:

Entity 1, PICK1 89 residues - Formula weight is not available

1   SERMETTHRVALPROGLYLYSVALTHRLEU
2   GLNLYSASPALAGLNASNLEUILEGLYILE
3   SERILEGLYGLYGLYALAGLNTYRCYSPRO
4   CYSLEUTYRILEVALGLNVALPHEASPASN
5   THRPROALAALALEUASPGLYTHRVALALA
6   ALAGLYASPGLUILETHRGLYVALASNGLY
7   ARGSERILELYSGLYLYSTHRLYSVALGLU
8   VALALALYSMETILEGLNGLUVALLYSGLY
9   GLUVALTHRILEHISTYRASNLYSLEU

Samples:

sample_1: PICK1 PDZ domain for alpha7nAChR binding, [U-100% 15N], 70 ± 5 uM; TRIS 25 mM; sodium chloride 125 mM; DSS 20 uM; LDAO 1.5 mM

sample_conditions_1: ionic strength: 125 mM; pH: 7.4; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection

NMRPipe v10.9 - processing

NMRFAM-SPARKY v1.470 - data analysis

SPARKY v3.190 - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks