BMRB Entry 5222

Title:
1H, 15N and 13C resonance assignments and secondary structure of Tryparedoxin-I from Crithidia fasciculata
Deposition date:
2001-12-03
Original release date:
2002-08-22
Authors:
Krumme, Dirk; Hecht, Hans-Jurgen; Menge, Ulrich; Ross, Anton; Wray, Victor; Flohe, Leopold
Citation:

Citation: Krumme, Dirk; Hecht, Hans-Jurgen; Menge, Ulrich; Ross, Anton; Wray, Victor; Flohe, Leopold. "Letter to the Editor: 1H, 15N and 13C resonance assignments and secondary structure of Tryparedoxin-I from Crithidia fasciculata"  J. Biomol. NMR 22, 375-376 (2002).
PubMed: 12018487

Assembly members:

Assembly members:
tryparedoxin I, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Crithidia fasciculata   Taxonomy ID: 5656   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Crithidia fasciculata

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b(+)

Data sets:
Data typeCount
13C chemical shifts404
15N chemical shifts133
1H chemical shifts824

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tryparedoxin I1

Entities:

Entity 1, tryparedoxin I 153 residues - Formula weight is not available

1   SERGLYLEUASPLYSTYRLEUPROGLYILE
2   GLULYSLEUARGARGGLYASPGLYGLUVAL
3   GLUVALLYSSERLEUALAGLYLYSLEUVAL
4   PHEPHETYRPHESERALASERTRPCYSPRO
5   PROCYSARGGLYPHETHRPROGLNLEUILE
6   GLUPHETYRASPLYSPHEHISGLUSERLYS
7   ASNPHEGLUVALVALPHECYSTHRTRPASP
8   GLUGLUGLUASPGLYPHEALAGLYTYRPHE
9   ALALYSMETPROTRPLEUALAVALPROPHE
10   ALAGLNSERGLUALAVALGLNLYSLEUSER
11   LYSHISPHEASNVALGLUSERILEPROTHR
12   LEUILEGLYVALASPALAASPSERGLYASP
13   VALVALTHRTHRARGALAARGALATHRLEU
14   VALLYSASPPROGLUGLYGLUGLNPHEPRO
15   TRPLYSASPALAPROLEUGLUHISHISHIS
16   HISHISHIS

Samples:

sample_1: tryparedoxin I, [U-13C; U-15N], 1 mM

sample_conditions_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HAHB(CO)HNnot availablenot availablenot available
15N-edited TOCSYnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

XWINNMR v1.3 - aquisition, processing

Aurelia v2.7.9 - assignments

NMR spectrometers:

  • Bruker AvanceDMX 600 MHz
  • Varian UnityINOVA 750 MHz

Related Database Links:

PDB
GenBank AAC72299 AAD20445

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks