BMRB Entry 52170

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52170

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Title:
1H, 13C and 15N chemical shift assignment of PhoSL (Pholiota squarrosa Lectin)
Deposition date:
2023-10-12
Original release date:
2023-12-01
Authors:
Lou, Yuan-Chao; Chou, Chun-Chi; Hsu, Chun-Hua
Citation:

Citation: Lou, Yuan-Chao; Tu, Cheng-Fen; Chou, Chun-Chi; Yeh, Hsin-Hong; Chien, Chia-Yu; Sadotra, Sushant; Chen, Chinpan; Yang, Ruey-Bing; Hsu, Chun-Hua. "Structural insights into the role of N-terminal integrity in PhoSL for core-fucosylated N-glycan recognition"  Int. J. Biol. Macromol. 255, 128309-128309 (2023).
PubMed: 37995778

Assembly members:

Assembly members:
entity_1, polymer, 40 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pholiota squarrosa   Taxonomy ID: 75321   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Pholiota squarrosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: APVPVTKLVCDGDTYKCTAY LDFGDGRWVAQWDTNVFHTG

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts41
1H chemical shifts267

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PhoSL, chain 11
2PhoSL, chain 21
3PhoSL, chain 31

Entities:

Entity 1, PhoSL, chain 1 40 residues - Formula weight is not available

1   ALAPROVALPROVALTHRLYSLEUVALCYS
2   ASPGLYASPTHRTYRLYSCYSTHRALATYR
3   LEUASPPHEGLYASPGLYARGTRPVALALA
4   GLNTRPASPTHRASNVALPHEHISTHRGLY

Samples:

sample_1: PhoSL (Pholiota squarrosa Lectin), [U-99% 13C; U-99% 15N], 1.2 mM; D2O, [U-99% 2H], 10%; H2O 90%; sodium phosphate 20 mM; sodium chloride 30 mM; sodium azide 1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1 - collection

NMRPipe - processing

NMRView - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks