BMRB Entry 52160

Name: MRE11 565_595
Published: 2023-12-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52160

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

MRE11 565_595

Deposition date:

2023-10-02

Original release date:

2023-12-27

Authors:

Usluer, Sinem; Madl, Tobias

Citation:

Citation: Usluer, Sinem; Galhuber, Markus; Khanna, Yukti; Bourgeois, Benjamin; Spreitzer, Emil; Michenthaler, Helene; Prokesch, Andreas; Madl, Tobias. "Disordered regions mediate the interaction of p53 and MRE11" Biochim. Biophys. Acta Mol. Cell Res. 1871, 119654-119654 (2023).
PubMed: 38123020

Assembly members:

Assembly members:
entity_1, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TNKGRGRGRGRRGGRGQNSA SRGGSQRGRA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	27
15N chemical shifts	23
1H chemical shifts	22

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MRE11	1

Entities:

Entity 1, MRE11 30 residues - Formula weight is not available

1	THR	ASN	LYS	GLY	ARG	GLY	ARG	GLY	ARG	GLY
2	ARG	ARG	GLY	GLY	ARG	GLY	GLN	ASN	SER	ALA
3	SER	ARG	GLY	GLY	SER	GLN	ARG	GLY	ARG	ALA

Samples:

sample_1: MRE11, [U-99% 15N], 300 uM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 298K K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HN(CAN)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	THR	ASN	LYS	GLY	ARG	GLY	ARG	GLY	ARG	GLY
2	ARG	ARG	GLY	GLY	ARG	GLY	GLN	ASN	SER	ALA
3	SER	ARG	GLY	GLY	SER	GLN	ARG	GLY	ARG	ALA

1	THR	ASN	LYS	GLY	ARG	GLY	ARG	GLY	ARG	GLY
2	ARG	ARG	GLY	GLY	ARG	GLY	GLN	ASN	SER	ALA
3	SER	ARG	GLY	GLY	SER	GLN	ARG	GLY	ARG	ALA

1	THR	ASN	LYS	GLY	ARG	GLY	ARG	GLY	ARG	GLY
2	ARG	ARG	GLY	GLY	ARG	GLY	GLN	ASN	SER	ALA
3	SER	ARG	GLY	GLY	SER	GLN	ARG	GLY	ARG	ALA