BMRB Entry 52150

Name: Solution structure of thioredoxin from Pisum sativum
Published: 2024-02-08

Click here to enlarge.

PDB ID: 8qon
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52150
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of thioredoxin from Pisum sativum

Deposition date:

2023-09-26

Original release date:

2024-02-08

Authors:

Neira, Jose Luis

Citation:

Citation: Neira, Jose; Palomino-Schatzlein, Martina; Rejas, Virginia; Traverso, Jose; Rico, Manual; Lopez-Gorge, Julio; Chueca, Ana; Camara-Artigas, Ana. "Three-dimensional solution structure, dynamics and binding of thioredoxin m from Pisum sativum" Int. J. Biol. Macromol. ., 129781-129781 (2024).
PubMed: 38296131

Assembly members:

Assembly members:
entity_1, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: garden pea Taxonomy ID: 3888 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pisum sativum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETm11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AVNEVQVVNDSSWDELVIGS ETPVLVDFWAPWCGPCRMIA PIIDELAKEYAGKIKCYKLN TDESPNTATKYGIRSIPTVL FFKNGEKKDSVIGAVPKATL SEKVEKYI

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	304
15N chemical shifts	112
1H chemical shifts	723

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Thioredoxin	1

Entities:

Entity 1, Thioredoxin 108 residues - Formula weight is not available

Ala is the first. Ile is the last

1

ALA

VAL

ASN

GLU

VAL

GLN

VAL

ASN

ASP

2

SER

TRP

ASP

GLU

LEU

VAL

ILE

GLY

SER

3

GLU

THR

PRO

VAL

LEU

VAL

ASP

PHE

TRP

ALA

4

PRO

TRP

CYS

GLY

PRO

CYS

ARG

MET

ILE

ALA

5

PRO

ILE

ASP

GLU

LEU

ALA

LYS

GLU

TYR

6

ALA

GLY

LYS

ILE

LYS

CYS

TYR

LYS

LEU

ASN

7

THR

ASP

GLU

SER

PRO

ASN

THR

ALA

THR

LYS

8

TYR

GLY

ILE

ARG

SER

ILE

PRO

THR

VAL

LEU

9

PHE

LYS

ASN

GLY

GLU

LYS

ASP

SER

10

VAL

ILE

GLY

ALA

VAL

PRO

LYS

ALA

THR

LEU

11

SER

GLU

LYS

VAL

GLU

LYS

TYR

ILE

Samples:

sample_1: Thioredoxin 2 mM; Thioredoxin, [U-100% 13C; U-100% 15N], 2 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.050 M; pH: 5.8; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2d noesy	sample_1	isotropic	sample_conditions_1
2d tocsy	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

TOPSPIN v2.1 - acquisition

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks