BMRB Entry 52147

Name: RNA binding protein hMEX3B can specifically recognize HLA-A mRNA to promote tumor immune escape
Published: 2023-10-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52147

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RNA binding protein hMEX3B can specifically recognize HLA-A mRNA to promote tumor immune escape

Deposition date:

2023-09-25

Original release date:

2023-10-25

Authors:

Yang, Kanglong; Zhang, Liang; Zhang, Jiahai

Citation:

Citation: Yang, Kanglong; Chen, Guanglin; Yu, Fan; Fang, Xianyang; Zhang, Jiahai; Zhang, Zhiyong; Shi, Yunyu; Zhang, Liang. "Molecular mechanism of specific HLA-A mRNA recognition by the RNA-binding-protein hMEX3B to promote tumor immune escape" Commun. Biol. 7, 158-158 (2024).
PubMed: 38326406

Assembly members:

Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DSEPRKKSVNMTECVPVPSS EHVAEIVGRQGCKIKALRAK TNTYIKTPVRGEEPVFVVTG RKEDVAMARREIISAAEHFS MIRASRNKN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	361
15N chemical shifts	89
1H chemical shifts	573

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MEX3B KH1 domain	1

Entities:

Entity 1, MEX3B KH1 domain 89 residues - Formula weight is not available

1

ASP

SER

GLU

PRO

ARG

LYS

SER

VAL

ASN

2

MET

THR

GLU

CYS

VAL

PRO

VAL

PRO

SER

3

GLU

HIS

VAL

ALA

GLU

ILE

VAL

GLY

ARG

GLN

4

GLY

CYS

LYS

ILE

LYS

ALA

LEU

ARG

ALA

LYS

5

THR

ASN

THR

TYR

ILE

LYS

THR

PRO

VAL

ARG

6

GLY

GLU

PRO

VAL

PHE

VAL

THR

GLY

7

ARG

LYS

GLU

ASP

VAL

ALA

MET

ALA

ARG

8

GLU

ILE

SER

ALA

GLU

HIS

PHE

SER

9

MET

ILE

ARG

ALA

SER

ARG

ASN

LYS

ASN

Samples:

sample_1: MEX3B KH1 domain, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 50 mM; NaCl 100 mM; TCEP 5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

Topspin4.09,CYANA - data collect, chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks