BMRB Entry 52108

Title:
Backbone 1H and 15N chemical shift assignments of K29-linked diubiquitin
Deposition date:
2023-08-31
Original release date:
2023-09-05
Authors:
Kurbah, Iladeiti; Castaneda, Carlos; Fushman, David
Citation:

Citation: Kurbah, Iladeiti; Castaneda, Carlos; Fushman, David. "TBD"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, 8564.84 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts139
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin, Chain A1
2ubiquitin, Chain B1

Entities:

Entity 1, ubiquitin, Chain A 76 residues - 8564.84 Da.

K29-linked diubiquitin

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: ubiquitin, Chain A, [U-99% 15N], 150 uM; ubiquitin, Chain B 150 uM; sodium phosphate 20 mM

sample_2: ubiquitin, Chain A 150 uM; ubiquitin, Chain B, [U-99% 15N], 150 uM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.470 - chemical shift assignment, peak picking

TOPSPIN v4.1.4 - processing of raw NMR data

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Related Database Links:

UNP P0CG47

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks