BMRB Entry 52103

Title:
Backbone assignment of SPIN-cys-aureus
Deposition date:
2023-08-25
Original release date:
2024-05-21
Authors:
Mishra, Nitin; Geisbrecht, Brian; Prakash, Om
Citation:

Citation: Fatehi, Soheila; Mishra, Nitin; Herdendorf, Timothy; Prakash, Om; Geisbrecht, Brian. "Staphylococcal peroxidase inhibitor (SPIN): Investigation of the inhibitory N-terminal domain via a stabilizing disulfide insertion"  Arch. Biochem. Biophys. 758, 110060-110060 (2024).
PubMed: 38880318

Assembly members:

Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7HMT

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts104
15N chemical shifts73
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SPIN-cys-aureus1

Entities:

Entity 1, SPIN-cys-aureus 73 residues - Formula weight is not available

1   LYSCYSTYRSERGLNASNGLYLEUVALLEU
2   HISCYSASPALAASNPHELEUGLUHISGLU
3   LEUSERTYRILEASPVALLEULEUASPLYS
4   ASNALAASPGLNALATHRLYSASPASNLEU
5   ARGSERTYRPHEALAASPLYSGLYLEUHIS
6   SERILELYSASPILEILEASNLYSALALYS
7   GLNASPGLYPHEASPVALSERLYSTYRGLU
8   HISVALLYS

Samples:

sample_1: SPIN-cys-aureus, [U-100% 13C; U-100% 15N], 0.8 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1 - collection

NMRPipe - processing

CARA v1.9.1.7 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks