BMRB Entry 52099

Title:
backbone 13C and 15N chemical shift assignment of the conductance domain of Influenza A M2 protein in DPhPC proteoliposomes
Deposition date:
2023-08-24
Original release date:
2023-11-15
Authors:
Mohr, Swantje; Lange, Adam
Citation:

Citation: Paschke, Ronja; Mohr, Swantje; Lange, Sascha; Lange, Adam; Kozuch, Jacek. "In Situ Spectroscopic Detection of Large-Scale Reorientations of Transmembrane Helices During Influenza A M2 Channel Opening"  Angew. Chem., Int. Ed. 62, e202309069-e202309069 (2023).
PubMed: 37733579

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, 5000 Da.

Natural source:

Natural source:   Common Name: Haemophilus influenzae   Taxonomy ID: 727   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RSNDSSDPLVVAASIIGILH LILWILDRLFFKSIYRFFEH GLK

Data sets:
Data typeCount
13C chemical shifts98
15N chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1M2 monomer A1
2M2 monomer B1

Entities:

Entity 1, M2 monomer A 43 residues - 5000 Da.

1   ARGSERASNASPSERSERASPPROLEUVAL
2   VALALAALASERILEILEGLYILELEUHIS
3   LEUILELEUTRPILELEUASPARGLEUPHE
4   PHELYSSERILETYRARGPHEPHEGLUHIS
5   GLYLEULYS

Samples:

sample_1: M2 conductance domain, [U-100% 13C; U-100% 15N], 50 % w/w; sodium phosphate 40 mM; glutamate 30 mM; sodium azide 3 mM; 1,2-diphytanoyl-sn-glycero-3-phosphocholine 50 % w/w

sample_conditions_1: pH: 7.8; pressure: 1 atm; temperature: 387 K

Experiments:

NameSampleSample stateSample conditions
3D (H)NCACBsample_1isotropicsample_conditions_1
3D (H)NCACOsample_1isotropicsample_conditions_1
3D (H)NCOCAsample_1isotropicsample_conditions_1
3D (H)CANCOsample_1isotropicsample_conditions_1
2D (H)NCACXsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1.1 - data collection, initial spectra representation

CcpNMR v3.1.0 - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz