BMRB Entry 52075

Name: NMR Assignments and Structure for the Dimeric Kinesin Neck Domain
Published: 2023-10-29

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PDB ID: 8tt7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52075
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignments and Structure for the Dimeric Kinesin Neck Domain

Deposition date:

2023-08-08

Original release date:

2023-10-29

Authors:

Seo, Diana; Kammerer, Richard; Alexandrescu, Andrei

Citation:

Citation: Seo, Diana; Kammerer, Richard; Alexandrescu, Andrei. "Solution NMR assignments and structure for the dimeric kinesin neck domain" Biomol. NMR Assign. 17, 301-307 (2023).
PubMed: 37861970

Assembly members:

Assembly members:
entity_1, polymer, 54 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHisTrx2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKTIKNTVSVNLELTAEEW KKKYEKEKEKNKALKSVIQH LEVELNRWRNGEAV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	243
15N chemical shifts	61
1H chemical shifts	400

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Kinesin Neck Domain, Chain A	1
2	Kinesin Neck Domain, Chain B	1

Entities:

Entity 1, Kinesin Neck Domain, Chain A 54 residues - Formula weight is not available

1

GLY

SER

LYS

THR

ILE

LYS

ASN

THR

VAL

SER

2

VAL

ASN

LEU

GLU

LEU

THR

ALA

GLU

TRP

3

LYS

TYR

GLU

LYS

GLU

LYS

GLU

LYS

4

ASN

LYS

ALA

LEU

LYS

SER

VAL

ILE

GLN

HIS

5

LEU

GLU

VAL

GLU

LEU

ASN

ARG

TRP

ARG

ASN

6

GLY

GLU

ALA

VAL

Samples:

sample_1: Kinesin Neck Domain, [U-99% 13C; U-99% 15N], 0.75 mM; NaCl 150 mM

sample_2: Kinesin Neck Domain, [U-99% 13C; U-99% 15N], 0.75 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.1; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HCACO	sample_2	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Software:

ANALYSIS - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Agilent INOVA 600 MHz
Bruker AVANCE NEO 600 MHz
Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks