BMRB Entry 52071

Title:
Backbone assignment of Ca2+ bound Arabidopsis thaliana Calmodulin1
Deposition date:
2023-08-07
Original release date:
2023-09-30
Authors:
Pedretti, Marco; Favretto, Filippo; Troilo, Francesca; Giovannoni, Moira; Conter, Carolina; Mattei, Benedetta; Dominici, Paola; Travaglini-Allocatelli, Carlo; Di Matteo, Adele; Astegno, Alessandra
Citation:

Citation: Pedretti, Marco; Favretto, Filippo; Troilo, Francesca; Giovannoni, Moira; Conter, Carolina; Mattei, Benedetta; Dominici, Paola; Travaglini-Allocatelli, Carlo; Di Matteo, Adele; Astegno, Alessandra. "Role of myristoylation in modulating PCaP1 interaction with calmodulin"  Plant Physiol. Biochem. 203, 108003-108003 (2023).
PubMed: 37717348

Assembly members:

Assembly members:
entity_1, polymer, 149 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET12b

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts144
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AtCaM1 calcium bound1
2calcium ion, 12
3calcium ion, 22
4calcium ion, 32
5calcium ion, 42

Entities:

Entity 1, AtCaM1 calcium bound 149 residues - Formula weight is not available

1   METALAASPGLNLEUTHRASPGLUGLNILE
2   SERGLUPHELYSGLUALAPHESERLEUPHE
3   ASPLYSASPGLYASPGLYCYSILETHRTHR
4   LYSGLULEUGLYTHRVALMETARGSERLEU
5   GLYGLNASNPROTHRGLUALAGLULEUGLN
6   ASPMETILEASNGLUVALASPALAASPGLY
7   ASNGLYTHRILEASPPHEPROGLUPHELEU
8   ASNLEUMETALALYSLYSMETLYSASPTHR
9   ASPSERGLUGLUGLULEULYSGLUALAPHE
10   ARGVALPHEASPLYSASPGLNASNGLYPHE
11   ILESERALAALAGLULEUARGHISVALMET
12   THRASNLEUGLYGLULYSLEUTHRASPGLU
13   GLUVALGLUGLUMETILEARGGLUALAASP
14   VALASPGLYASPGLYGLNILEASNTYRGLU
15   GLUPHEVALLYSILEMETMETALALYS

Entity 2, calcium ion, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: AtCaM1 Ca2+, [U-100% 13C; U-100% 15N], 1.6 mM; KCl 50 mM; TRIS 50 mM; DTT 0.5 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.5.2 - chemical shift assignment, peak picking

NMRPipe v9.9 - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks