BMRB Entry 52054

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52054
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
HsPEX14 237-245
Deposition date:
2023-07-24
Original release date:
2026-10-01
Authors:
Emmanouilidis, Leonidas; Gaussmann, Stefan; Sattler, Michael
Citation:

Citation: Emmanouilidis, Leonidas. "Gatekeeping of peroxisomal import by an allosteric modulation of receptor/cargo interactions "  Nature Communications ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 9 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KIPSWQIPV

Data sets:
Data typeCount
13C chemical shifts40
15N chemical shifts9
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PEX14 237-2451

Entities:

Entity 1, PEX14 237-245 9 residues - Formula weight is not available

1   LYSILEPROSERTRPGLNILEPROVAL

Samples:

sample_1: PEX14 237-245, [U-99% 13C; U-99% 15N], 0.75 mM; sodium chloride 50 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 83 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6 - collection

NMRPipe - processing

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 600 MHz

Related Database Links:

UNP O75381
AlphaFold O75381

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks