BMRB Entry 5200

Title:
Sequence-specific 1H, 13C and 15N chemical shift assignment of DnaA domain IV
Deposition date:
2001-11-04
Original release date:
2003-06-10
Authors:
Imoto, Taiji
Citation:

Citation: Obita, Takayuki; Iwura, Takafumi; Su'etsugu, Masayuki; Yoshida, Yoichiro; Tanaka, Yoshitsugu; Katayama, Tsutomu; Ueda, Tadashi; Imoto, Taiji. "Determination of the Secondary Structure in Solution of the Escherichia coli DnaA DNA-binding Domain "  Biochem. Biophys. Res. Commun. 299, 42-48 (2002).
PubMed: 12435387

Assembly members:

Assembly members:
chromosome replication initiator, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts165
13C chemical shifts274
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DnaA domain IV1

Entities:

Entity 1, DnaA domain IV 95 residues - Formula weight is not available

1   METVALTHRILEASPASNILEGLNLYSTHR
2   VALALAGLUTYRTYRLYSILELYSVALALA
3   ASPLEULEUSERLYSARGARGSERARGSER
4   VALALAARGPROARGGLNMETALAMETALA
5   LEUALALYSGLULEUTHRASNHISSERLEU
6   PROGLUILEGLYASPALAPHEGLYGLYARG
7   ASPHISTHRTHRVALLEUHISALACYSARG
8   LYSILEGLUGLNLEUARGGLUGLUSERHIS
9   ASPILELYSGLUASPPHESERASNLEUILE
10   ARGTHRLEUSERSER

Samples:

sample_1: chromosome replication initiator 2.0 mM

Ex-cond_1: pH: 6.0; temperature: 303 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available

Software:

NMRPipe -

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAB38060 BAE73276 BAE77592 BAG79512 BAH65893
EMBL CAD03157 CAE12296 CAG77337 CAH23181 CAL14189
GB AAA02815 AAA02924 AAA83958 AAB59149 AAC76725
PIR AI0957
REF NP_290335 NP_312664 NP_418157 NP_458104 NP_462738
SP A1JT80 A4TGL5 A7FPB7 A7ZTQ8 A8A6G3
AlphaFold A1JT80 A4TGL5 A7FPB7 A7ZTQ8 A8A6G3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks