BMRB Entry 51996

Title:
DNAJB6 J-domain
Deposition date:
2023-06-11
Original release date:
2023-10-30
Authors:
Abayev-Avraham, Meital; Rosenzweig, Rina
Citation:

Citation: Abayev-Avraham, Meital; Salzberg, Yehuda; Gliksberg, Dar; Oren-Suissa, Meital; Rosenzweig, Rina. "DNAJB6 mutants display toxic gain of function through unregulated interaction with Hsp70 chaperones"  Nat. Commun. 14, 7066-7066 (2023).
PubMed: 37923706

Assembly members:

Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-sumo

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts135
15N chemical shifts68
1H chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNAJB6 JD1

Entities:

Entity 1, DNAJB6 JD 72 residues - Formula weight is not available

1   METVALASPTYRTYRGLUVALLEUGLYVAL
2   GLNARGHISALASERPROGLUASPILELYS
3   LYSALATYRARGLYSLEUALALEULYSTRP
4   HISPROASPLYSASNPROGLUASNLYSGLU
5   GLUALAGLUARGLYSPHELYSGLNVALALA
6   GLUALATYRGLUVALLEUSERASPALALYS
7   LYSARGASPILETYRASPLYSTYRGLYLYS
8   GLUGLY

Samples:

sample_1: DNAJB6 JD, [U-100% 13C; U-100% 15N], 3.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 1000 MHz
  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks