BMRB Entry 51978

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human PHOX2B C-terminal domain at 278K
Deposition date:
2023-05-26
Original release date:
2024-03-05
Authors:
Anton, Rosa; Trevino, Miguel; Pantoja-Uceda, David
Citation:

Citation: Anton, Rosa; Trevino, Miguel A.; Pantoja-Uceda, David; Felix, Sara; Babu, Maria; Cabrita, Eurico J.; Zweckstetter, Markus; Tinnefeld, Philip; Vera, Andres M.; Oroz, Javier. "Alternative low-populated conformations prompt phase transitions in polyalanine repeat expansions"  Nat. Commun. 15, 1925-1925 (2024).
PubMed: 38431667

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts67
1H chemical shifts415

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human PHOX2B C-terminal domain1

Entities:

Entity 1, human PHOX2B C-terminal domain 87 residues - Formula weight is not available

1   GLYPROGLYGLYPROGLYGLYGLUPROGLY
2   LYSGLYGLYALAALAALAALAALAALAALA
3   ALAALAALAALAALAALAALAALAALAALA
4   ALAALAALAGLYGLYLEUALAALAALAGLY
5   GLYPROGLYGLNGLYTRPALAPROGLYPRO
6   GLYPROILETHRSERILEPROASPSERLEU
7   GLYGLYPROPHEALASERVALLEUSERSER
8   LEUGLNARGPROASNGLYALALYSALAALA
9   LEUVALLYSSERSERMETPHE

Samples:

sample_1: human PHOX2B C-terminal domain, [U-99% 13C; U-99% 15N], 0.5 mM; potassium phosphate 5 mM; sodium chloride 10 mM; beta-mercaptoethanol 2 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks