BMRB Entry 51947

Name: Backbone and partial side-chain assignment of the TAD domain (366-400) of human WW domain-containing transcription regulator protein 1 (TAZ/WWTR1)
Published: 2025-04-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51947

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and partial side-chain assignment of the TAD domain (366-400) of human WW domain-containing transcription regulator protein 1 (TAZ/WWTR1)

Deposition date:

2023-04-27

Original release date:

2025-04-01

Authors:

Zhang, Haoran; Li, Yan

Citation:

Citation: Zhang, Haoran; Li, Yan. "Backbone and partial side-chain assignment of the TAD domain (366-400) of human WW domain-containing transcription regulator protein 1 (TAZ/WWTR1)" .

Assembly members:

Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSGTNVDLGTLESEDLIPLF NDVESALNKSEPFLTWL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	105
15N chemical shifts	36
1H chemical shifts	138

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TAZ TAD domain (366-400)	1

Entities:

Entity 1, TAZ TAD domain (366-400) 37 residues - Formula weight is not available

The recombinant TAZ TAD domain (366-400) was expressed using a pET-22b plasmid incorporated with SUMO tag for improved protein expression, with SUMO cut later for NMR characterization. TAZ TAD domain (366-400) begins with sequence GTN..., and the upstream GS is supposed to be the remaining SUMO tag.

1

GLY

SER

GLY

THR

ASN

VAL

ASP

LEU

GLY

THR

2

LEU

GLU

SER

GLU

ASP

LEU

ILE

PRO

LEU

PHE

3

ASN

ASP

VAL

GLU

SER

ALA

LEU

ASN

LYS

SER

4

GLU

PRO

PHE

LEU

THR

TRP

LEU

Samples:

sample_1: TAZ TAD domain (366-400), [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 5%; TCEP 0.5 mM

sample_conditions_1: ionic strength: 170 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe v11.1 - processing

POKY vBuild 20220114 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks