BMRB Entry 51940

Title:
Backbone Assignment of Human Polymerase Theta MOTIF 2 phosphorylated by PLK1
Deposition date:
2023-04-26
Original release date:
2023-10-03
Authors:
Zinn-Justin, Sophie; Miron, Simona; Boeffard-Dosierre, Liza; Airs-Loeuilliette, Julie
Citation:

Citation: Gelot, Camille; Kovacs, Marton Tibor; Miron, Simona; Mylne, Emilie; Haan, Alexis; Boeffard-Dosierre, Liza; Ghouil, Rania; Popova, Tatiana; Dingli, Florent; Loew, Damarys; Guirouilh-Barbat, Josee; Del Nery, Elaine; Zinn-Justin, Sophie; Ceccaldi, Raphael. "Polth is phosphorylated by PLK1 to repair double-strand breaks in mitosis"  Nature 621, 415-422 (2023).
PubMed: 37674080

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Data sets:
Data typeCount
13C chemical shifts340
15N chemical shifts111
1H chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Polymerase Theta MOTIF 2 phosphorylated by PLK11

Entities:

Entity 1, Human Polymerase Theta MOTIF 2 phosphorylated by PLK1 121 residues - Formula weight is not available

1   GLUASNGLNASPTHRHISGLNGLNLEUTHR
2   GLNSEPASNASPGLUSEPILEILEPHESER
3   GLUMETASPSEPVALGLNMETVALGLUALA
4   LEUASPASNVALASPILEPHEPROVALGLN
5   GLULYSASNHISTPOVALVALSERPROARG
6   ALALEUGLULEUSEPASPPROVALLEUASP
7   GLUHISHISGLNGLYASPGLNASPGLYGLY
8   ASPGLNASPGLUARGALAGLULYSSERLYS
9   LEUTHRGLYTHRARGGLNASNHISSEPPHE
10   ILETRPSERGLYALASEPPHEASPLEUSER
11   PROGLYLEUGLNARGILELEUASPLYSVAL
12   SERSERPROLEUGLUASNGLULYSLEULYS
13   SER

Samples:

sample_1: Human Polymerase Theta MOTIF 2 phosphorylated by PLK1, [U-95% 13C; U-95% 15N], 300 uM

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCANNHsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks