BMRB Entry 51929

Name: Backbone resonance assignments for dengue 4 mini-NS2B in detergent micelles
Published: 2023-08-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51929

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments for dengue 4 mini-NS2B in detergent micelles

Deposition date:

2023-04-19

Original release date:

2023-08-30

Authors:

Loh, Yingru; Kang, Congbao

Citation:

Citation: Li, Qingxin; Ng, Hui Qi; Loh, Ying Ru; Kang, CongBao. "Backbone 1H, 15N and 13C resonance assignments for dengue NS2B without the NS3 protease cofactor region in detergent micelles" Biomol. NMR Assign. 17, 205-209 (2023).
PubMed: 37405583

Assembly members:

Assembly members:
entity_1, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source: Common Name: dengue virus Taxonomy ID: 12637 Superkingdom: Viruses Kingdom: not available Genus/species: Flavivirus dengue virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNEGIMAVGLVSLLGSALLK NDVPLAGPMVAGGLLLAAYV MSGSSEETNMITLLVKLALI TVSGLYPLAIPVTMTLWYMW QVKTQRHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	247
15N chemical shifts	75
1H chemical shifts	267

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NS2B	1

Entities:

Entity 1, NS2B 92 residues - Formula weight is not available

The residues 46-86 of NS2B are not included in this mini-NS2B construct.

1

MET

ASN

GLU

GLY

ILE

MET

ALA

VAL

GLY

LEU

2

VAL

SER

LEU

GLY

SER

ALA

LEU

LYS

3

ASN

ASP

VAL

PRO

LEU

ALA

GLY

PRO

MET

VAL

4

ALA

GLY

LEU

ALA

TYR

VAL

5

MET

SER

GLY

SER

GLU

THR

ASN

MET

6

ILE

THR

LEU

VAL

LYS

LEU

ALA

LEU

ILE

7

THR

VAL

SER

GLY

LEU

TYR

PRO

LEU

ALA

ILE

8

PRO

VAL

THR

MET

THR

LEU

TRP

TYR

MET

TRP

9

GLN

VAL

LYS

THR

GLN

ARG

HIS

10

HIS

Samples:

sample_1: Mini-NS2B, [U-100% 13C; U-100% 15N], 0.45 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1 - collection

NMRPipe - processing

NMRView - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks