BMRB Entry 51871

Title:
1H, 13C, and 15N backbone NMR resonance assignments of TANGO1 (30-139) from Drosophila melanogaster
Deposition date:
2023-03-07
Original release date:
2023-03-08
Authors:
Arnolds, Oliver; Stoll, Raphael
Citation:

Citation: Arnolds, Oliver; Stoll, Raphael. "Characterization of a fold in TANGO1 evolved from SH3 domains for the export of bulky cargos"  Nat. Commun. 14, 2273-2273 (2023).
PubMed: 37080980

Assembly members:

Assembly members:
entity_1, polymer, 111 residues, 12115.1615 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE40

Data sets:
Data typeCount
13C chemical shifts327
15N chemical shifts108
1H chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TANGO DROME1

Entities:

Entity 1, TANGO DROME 111 residues - 12115.1615 Da.

1   METTHRLEUTHRTRPALAALATHRLEUSER
2   ASPLYSARGLEUCYSALAASPPROLYSCYS
3   GLUGLNILEILESERMETGLYILEALALYS
4   ILETHRTYRALAILEGLYGLYGLUGLYLEU
5   ILESERPHELYSILEASNSERPROILEARG
6   VALLEUSERLYSSERALAGLYSERASNMET
7   GLNLEUTRPGLYVALASPILEASNGLYARG
8   ARGGLYTYRALAASNLYSASPPHEILEMET
9   GLULYSLYSILELEUVALARGASPLYSASP
10   LEULEUTYRGLUVALPROVALVALGLYPRO
11   GLYSERPROVALGLNSERVALGLUTHRPRO
12   VAL

Samples:

sample_1: TANGO_DROME, [U-100% 13C; U-100% 15N], 1.0000 mM; sodium azide 0.0002 kg/m3; NaCl 137.0000 mM; KCl 2.7000 mM; Disodiumhydrogenphosphate 10.0000 mM; Potassium dihydrogen phosphate 1.8000 mM

sample_conditions_1: ionic strength: 166.500 mM; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
HNcoCACB (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
HNcaCO (H[N[ca[CO]]])sample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
hCCcoNH (hC_cacoNH.relayed)sample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker DRX 600 MHz
  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks