BMRB Entry 51832

Title:
NMR Resonance Assignment of a Fibroblast Growth Factor 8 splicing isoform b.
Deposition date:
2023-02-16
Original release date:
2023-05-23
Authors:
Hargittay, Bruno; Mineev, Konstantin; Richter, Christian; Jonker, Hendrik; Saxena, Krishna; Schwalbe, Harald
Citation:

Citation: Hargittay, Bruno; Mineev, Konstantin; Richter, Christian; Sreeramulu, Sridhar; Jonker, Hendrik; Saxena, Krishna; Schwalbe, Harald. "NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b"  Biomol. NMR Assignments 17, 135-142 (2023).
PubMed: 37118562

Assembly members:

Assembly members:
entity_1, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKMProtG

Data sets:
Data typeCount
13C chemical shifts660
15N chemical shifts170
1H chemical shifts1112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FGF8b1

Entities:

Entity 1, FGF8b 168 residues - Formula weight is not available

1   GLYALAMETGLYGLNVALTHRVALGLNSER
2   SERPROASNPHETHRGLNHISVALARGGLU
3   GLNSERLEUVALTHRASPGLNLEUSERARG
4   ARGLEUILEARGTHRTYRGLNLEUTYRSER
5   ARGTHRSERGLYLYSHISVALGLNVALLEU
6   ALAASNLYSARGILEASNALAMETALAGLU
7   ASPGLYASPPROPHEALALYSLEUILEVAL
8   GLUTHRASPTHRPHEGLYSERARGVALARG
9   VALARGGLYALAGLUTHRGLYLEUTYRILE
10   CYSMETASNLYSLYSGLYLYSLEUILEALA
11   LYSSERASNGLYLYSGLYLYSASPCYSVAL
12   PHETHRGLUILEVALLEUGLUASNASNTYR
13   THRALALEUGLNASNALALYSTYRGLUGLY
14   TRPTYRMETALAPHETHRARGLYSGLYARG
15   PROARGLYSGLYSERLYSTHRARGGLNHIS
16   GLNARGGLUVALHISPHEMETLYSARGLEU
17   PROARGGLYHISHISTHRTHRGLU

Samples:

sample_1: FGF8b, [U-100% 13C; U-100% 15N], 400 uM; NaPi 50 mM; NaCl 300 mM

sample_conditions_1: ionic strength: 350 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1.1 - collection

SPARKY - chemical shift assignment

POKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz
  • Bruker AVANCE III 950 MHz
  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 600 MHz

Related Database Links:

SP P55075-3
AlphaFold P55075-3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks