BMRB Entry 51800

Name: 1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein
Published: 2023-06-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51800

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

Deposition date:

2023-01-24

Original release date:

2023-06-19

Authors:

Matosin, Srdan; Fischer, Patrick; Droemer, Maxim; Arthanari, Haribabu; Nagarajan, Rajesh

Citation:

Citation: Matosin, Srdan; Fischer, Patrick; Droemer, Maxim; Baggs, Eric; Chowdhury, Abu Sayeed; Tavares, Isidoro; Ficarro, Scott; Warner, Lisa Rose; Arthanari, Haribabu; Nagarajan, Rajesh. "1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein" Biomol. NMR Assign. 17, 167-171 (2023).
PubMed: 37233945

Assembly members:

Assembly members:
entity_1, polymer, 79 residues, Formula weight is not available
entity_PNS, non-polymer, 358.348 Da.

Natural source:

Natural source: Common Name: burkholderia mallei Taxonomy ID: 13373 Superkingdom: Bacteria Kingdom: not available Genus/species: burkholderia mallei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDNIEQRVKKIVAEQLGVAE AEIKNEASFVNDLGADSLDT VELVMALEDEFGMEIPDEEA EKITTVQQAIDYARANVKA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	316
15N chemical shifts	78
1H chemical shifts	501

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ACP	1
2	phosphopantetheine	2

Entities:

Entity 1, ACP 79 residues - Formula weight is not available

1

MET

ASP

ASN

ILE

GLU

GLN

ARG

VAL

LYS

2

ILE

VAL

ALA

GLU

GLN

LEU

GLY

VAL

ALA

GLU

3

ALA

GLU

ILE

LYS

ASN

GLU

ALA

SER

PHE

VAL

4

ASN

ASP

LEU

GLY

ALA

ASP

SER

LEU

ASP

THR

5

VAL

GLU

LEU

VAL

MET

ALA

LEU

GLU

ASP

GLU

6

PHE

GLY

MET

GLU

ILE

PRO

ASP

GLU

ALA

7

GLU

LYS

ILE

THR

VAL

GLN

ALA

ILE

8

ASP

TYR

ALA

ARG

ALA

ASN

VAL

LYS

ALA

Entity 2, phosphopantetheine - C11 H23 N2 O7 P S - 358.348 Da.

1

PNS

Samples:

sample_1: holoACPbm, [U-99% 13C; U-99% 15N], 1 mM; MES 50 mM; MgCl2 10 mM; TCEP-HCl 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.5 - data analysis

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks