BMRB Entry 51779

Name: Bacillus subtilis SepF protein assembly (wild type)
Published: 2023-02-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51779

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Bacillus subtilis SepF protein assembly (wild type)

Deposition date:

2023-01-10

Original release date:

2023-02-10

Authors:

Zhang, Chang; Liu, Wenjing

Citation:

Citation: Zhang, Chang; Liu, Wenjing; Deng, Jing; Ma, Shaojie; Chang, Ziwei; Yang, Jun. "Structural Insights into the Interaction between Bacillus subtilis SepF Assembly and FtsZ by Solid-State NMR Spectroscopy" J. Phys. Chem. B. 126, 5219-5230 (2022).
PubMed: 35799411

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSMKNKLKNFFSMEDEEYEY EYIETERESHEEHEQKEKPA YNGNKPAGKQNVVSLQSVQK SSKVVLSEPRVYAEAQEIAD HLKNRRAVVVNLQRIQHDQA KRIVDFLSGTVYAIGGDIQR IGSDIFLCTPDNVDVSGTIS ELISEDEHQRW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	310
15N chemical shifts	72

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SepF monomer	1

Entities:

Entity 1, SepF monomer 151 residues - Formula weight is not available

1

MET

SER

MET

LYS

ASN

LYS

LEU

LYS

ASN

PHE

2

PHE

SER

MET

GLU

ASP

GLU

TYR

GLU

TYR

3

GLU

TYR

ILE

GLU

THR

GLU

ARG

GLU

SER

HIS

4

GLU

HIS

GLU

GLN

LYS

GLU

LYS

PRO

ALA

5

TYR

ASN

GLY

ASN

LYS

PRO

ALA

GLY

LYS

GLN

6

ASN

VAL

SER

LEU

GLN

SER

VAL

GLN

LYS

7

SER

LYS

VAL

LEU

SER

GLU

PRO

ARG

8

VAL

TYR

ALA

GLU

ALA

GLN

GLU

ILE

ALA

ASP

9

HIS

LEU

LYS

ASN

ARG

ALA

VAL

10

ASN

LEU

GLN

ARG

ILE

GLN

HIS

ASP

GLN

ALA

11

LYS

ARG

ILE

VAL

ASP

PHE

LEU

SER

GLY

THR

12

VAL

TYR

ALA

ILE

GLY

ASP

ILE

GLN

ARG

13

ILE

GLY

SER

ASP

ILE

PHE

LEU

CYS

THR

PRO

14

ASP

ASN

VAL

ASP

VAL

SER

GLY

THR

ILE

SER

15

GLU

LEU

ILE

SER

GLU

ASP

GLU

HIS

GLN

ARG

16

TRP

Samples:

sample_1: SepF 57-151, [U-100% 13C; U-100% 15N], 38.9 % w/w

sample_conditions_1: pH: 8.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-detected NCA	sample_1	isotropic	sample_conditions_1
2D 13C-13C DARR	sample_1	isotropic	sample_conditions_1
NCACX	sample_1	isotropic	sample_conditions_1
NCOCX	sample_1	isotropic	sample_conditions_1
CONCA	sample_1	isotropic	sample_conditions_1
2D NcaCX	sample_1	isotropic	sample_conditions_1

Software:

VNMR - collection

NMRPipe - data analysis

SPARKY - chemical shift assignment

NMR spectrometers:

Varian Uniform NMR System 600 MHz