BMRB Entry 51748

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51748

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Title:
MERS Nsp9
Deposition date:
2022-12-19
Original release date:
2023-12-19
Authors:
Mani, Gayathri; El-Kamand, Serene; Cubeddu, Liza; Gamsjaeger, Roland
Citation:

Citation: Mani, Gayathri; El-Kamand, Serene; Wang, Bing; Baker, David; Ataide, Sandro; Artsimovitch, Irina; Cubeddu, Liza; Gamsjaeger, Roland. "A structural analysis of the nsp9 protein from the coronavirus MERS CoV reveals a conserved RNA binding interface"  Proteins 92, 418-426 (2024).
PubMed: 37929701

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: MERS coronavirus   Taxonomy ID: 1335626   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus MERS coronavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSF

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NNEIKPSGLKTMVVSAGQEQ TNCNTSSLAYYEPVQGRKML MALLSDNAYLKWARVEGKDG FVSVELQPPCKFLIAGPKGP EIRYLYFVKNLNNLHRGQVL GHIAATVRLQ

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts80
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MERS Nsp91

Entities:

Entity 1, MERS Nsp9 110 residues - Formula weight is not available

1   ASNASNGLUILELYSPROSERGLYLEULYS
2   THRMETVALVALSERALAGLYGLNGLUGLN
3   THRASNCYSASNTHRSERSERLEUALATYR
4   TYRGLUPROVALGLNGLYARGLYSMETLEU
5   METALALEULEUSERASPASNALATYRLEU
6   LYSTRPALAARGVALGLUGLYLYSASPGLY
7   PHEVALSERVALGLULEUGLNPROPROCYS
8   LYSPHELEUILEALAGLYPROLYSGLYPRO
9   GLUILEARGTYRLEUTYRPHEVALLYSASN
10   LEUASNASNLEUHISARGGLYGLNVALLEU
11   GLYHISILEALAALATHRVALARGLEUGLN

Samples:

sample_1: MERS Nsp9, [U-13C; U-15N], 1 mM; sodium phosphate 25 mM; NaCl 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 175 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks