BMRB Entry 51731

Title:
Cerato Ulmin
Deposition date:
2022-12-13
Original release date:
2023-03-07
Authors:
Gallo, Mariana; Spisni, Alberto; Pertinhez, Thelma
Citation:

Citation: Gallo, Mariana; Luti, Simone; Baroni, Fabio; Baccelli, Ivan; Cilli, Eduardo Maffud; Cicchi, Costanza; Leri, Manuela; Spisni, Alberto; Pertinhez, Thelma; Pazzagli, Luigia. "Plant Defense Elicitation by the Hydrophobin Cerato-Ulmin and Correlation with Its Structural Features"  Int. J. Mol. Sci. 24, 2251-2251 (2023).
PubMed: 36768573

Assembly members:

Assembly members:
entity_1, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Ophiostoma ulmi   Taxonomy ID: 5174   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Ophiostoma ulmi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: E. coli DH5alpha

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts64
1H chemical shifts323

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cerato-ulmin1

Entities:

Entity 1, cerato-ulmin 79 residues - Formula weight is not available

1   GLUALAGLUALASERASPSERTYRASPPRO
2   CYSTHRGLYLEULEUGLNLYSSERPROGLN
3   CYSCYSASNTHRASPILELEUGLYVALALA
4   ASNLEUASPCYSHISGLYPROPROSERVAL
5   PROTHRSERPROSERGLNPHEGLNALASER
6   CYSVALALAASPGLYGLYARGSERALAARG
7   CYSCYSTHRLEUSERLEULEUGLYLEUALA
8   LEUVALCYSTHRASPPROVALGLYILE

Samples:

sample_1: Cerato-ulmin, [U-100% 15N], 700 ± 20 uM; sodium phosphate 35 ± 3 mM; acetonitrile, [U-2H], 30 ± 5 %

sample_conditions_1: ionic strength: 38 mM; pH: 5.8; pressure: 1 atm; temperature: 288.0 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRViewJ v9.2 - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SP Q06153
AlphaFold Q06153

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks