BMRB Entry 51723

Title:
1H, 13C, 15N chemical shift assignments for soluble domain of Rieske iron-sulfur protein from Chlorobaculum tepidum
Deposition date:
2022-12-07
Original release date:
2023-05-24
Authors:
Kishimoto, Hiraku; Mutoh, Risa; Miyanoiri, Yohei; Tanaka, Hideaki; Kurisu, Genji; Oh-oka, Hirozo
Citation:

Citation: Kishimoto, Hiraku; Azai, Chihiro; Yamamoto, Tomoya; Mutoh, Risa; Nakaniwa, Tetsuko; Tanaka, Hideaki; Miyanoiri, Yohei; Kurisu, Genji; Oh-oka, Hirozo. "Soluble domains of cytochrome c-556 and Rieske iron-sulfur protein from Chlorobaculum tepidu m: Crystal structures and interaction analysis"  Curr. Res. Struct. Biol. 5, 100101-100101 (2023).
PubMed: 37180033

Assembly members:

Assembly members:
entity_1, polymer, 119 residues, 12798 Da.
entity_FES, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: green sulfur bacteria   Taxonomy ID: 1097   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Chlorobaculum tepidum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Data typeCount
13C chemical shifts1338
15N chemical shifts609
1H chemical shifts609

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rieske protein1
2[2Fe-2S]2

Entities:

Entity 1, Rieske protein 119 residues - 12798 Da.

Residues 182-194 is Histidine tag. This is the soluble domain (Residues 76-181) of a transmembrane protein.

1   ALALYSLYSILELYSILEVALASNGLULEU
2   ALAVALGLYPROALASERASPVALPROASN
3   GLYTHRGLYLYSILETYRGLNPHEASNASP
4   ASPLYSVALILEVALVALASNHISGLYGLY
5   SERLEUTHRALAVALSERALAILECYSTHR
6   HISLEUGLYCYSLEUVALHISTRPASPGLU
7   ALAALAASPMETILEALACYSPROCYSHIS
8   GLYALALYSTYRARGGLNASPGLYLYSILE
9   ILESERGLYPROGLNPROLEUPROLEULYS
10   GLNTYRLYSVALLYSILEGLUASPGLYLYS
11   ILEVALVALSERILEALALYSLEUALAALA
12   ALALEUGLUHISHISHISHISHISHIS

Entity 2, [2Fe-2S] - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: Rieske iron-sulfur protein, [U-13C; U-15N], 1.36 mM; TRIS 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks