BMRB Entry 51710

Title:
SARS-CoV-2 Phosphorylated Nucleocapsid 1-209 at 35C
Deposition date:
2022-11-26
Original release date:
2023-03-07
Authors:
Lee, Eunjeong
Citation:

Citation: Lee, Eunjeong; Redzic, Jasmina; Saviola, Anthony; Li, Xueni; Ebmeier, Christopher; Kutateladze, Tatiana; Hansen, Kirk Charles; Zhao, Rui; Ahn, Natalie; Sluchanko, Nikolai; Eisenmesser, Elan. "Molecular insight into the specific interactions of the SARS-CoV-2 Nucleocapsid with RNA and host protein"  Protein Sci 32, e4603-e4603 (2023).
PubMed: 36807437

Assembly members:

Assembly members:
entity_1, polymer, 209 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29

Data sets:
Data typeCount
13C chemical shifts185
15N chemical shifts166
1H chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SARS-Cov-2 Phosphorylated Nucleocapsid protein1

Entities:

Entity 1, SARS-Cov-2 Phosphorylated Nucleocapsid protein 209 residues - Formula weight is not available

1   METSERASPASNGLYPROGLNASNGLNARG
2   ASNALAPROARGILETHRPHEGLYGLYPRO
3   SERASPSERTHRGLYSERASNGLNASNGLY
4   GLUARGSERGLYALAARGSERLYSGLNARG
5   ARGPROGLNGLYLEUPROASNASNTHRALA
6   SERTRPPHETHRALALEUTHRGLNHISGLY
7   LYSGLUASPLEULYSPHEPROARGGLYGLN
8   GLYVALPROILEASNTHRASNSERSERPRO
9   ASPASPGLNILEGLYTYRTYRARGARGALA
10   THRARGARGILEARGGLYGLYASPGLYLYS
11   METLYSASPLEUSERPROARGTRPTYRPHE
12   TYRTYRLEUGLYTHRGLYPROGLUALAGLY
13   LEUPROTYRGLYALAASNLYSASPGLYILE
14   ILETRPVALALATHRGLUGLYALALEUASN
15   THRPROLYSASPHISILEGLYTHRARGASN
16   PROALAASNASNALAALAILEVALLEUGLN
17   LEUPROGLNGLYTHRTHRLEUPROLYSGLY
18   PHETYRALAGLUGLYSERARGGLYGLYSER
19   GLNALASERSERARGSERSERSERARGSER
20   ARGASNSERSERARGASNSERTHRPROGLY
21   SERSERARGGLYTHRSERPROALAARG

Samples:

sample_1: Sars-Cov-2 Nucleocapsid, [U-100% 15N], 500 uM; sodium chloride 150 mM; HEPES 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks