BMRB Entry 51709

Name: SARS-CoV-2 Nucleocapsid 251-419
Published: 2023-03-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51709

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SARS-CoV-2 Nucleocapsid 251-419

Deposition date:

2022-11-26

Original release date:

2023-03-07

Authors:

Lee, Eunjeong

Citation:

Citation: Lee, Eunjeong; Redzic, Jasmina; Saviola, Anthony; Li, Xueni; Ebmeier, Christopher; Kutateladze, Tatiana; Hansen, Kirk Charles; Zhao, Rui; Ahn, Natalie; Sluchanko, Nikolai; Eisenmesser, Elan. "Molecular insight into the specific interactions of the SARS-CoV-2 Nucleocapsid with RNA and host protein" Protein Sci 32, e4603-e4603 (2023).
PubMed: 36807437

Assembly members:

Assembly members:
entity_1, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AAEASKKPRQKRTATKAYNV TQAFGRRGPEQTQGNFGDQE LIRQGTDYKHWPQIAQFAPS ASAFFGMSRIGMEVTPSGTW LTYTGAIKLDDKDPNFKDQV ILLNKHIDAYKTFPPTEPKK DKKKKADETQALPQRQKKQQ TVTLLPAADLDDFSKQLQQS MSSADSTQA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	271
15N chemical shifts	128
1H chemical shifts	128

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nucleocapsid 251-419	1

Entities:

Entity 1, Nucleocapsid 251-419 169 residues - Formula weight is not available

1

ALA

GLU

ALA

SER

LYS

PRO

ARG

GLN

2

LYS

ARG

THR

ALA

THR

LYS

ALA

TYR

ASN

VAL

3

THR

GLN

ALA

PHE

GLY

ARG

GLY

PRO

GLU

4

GLN

THR

GLN

GLY

ASN

PHE

GLY

ASP

GLN

GLU

5

LEU

ILE

ARG

GLN

GLY

THR

ASP

TYR

LYS

HIS

6

TRP

PRO

GLN

ILE

ALA

GLN

PHE

ALA

PRO

SER

7

ALA

SER

ALA

PHE

GLY

MET

SER

ARG

ILE

8

GLY

MET

GLU

VAL

THR

PRO

SER

GLY

THR

TRP

9

LEU

THR

TYR

THR

GLY

ALA

ILE

LYS

LEU

ASP

10

ASP

LYS

ASP

PRO

ASN

PHE

LYS

ASP

GLN

VAL

11

ILE

LEU

ASN

LYS

HIS

ILE

ASP

ALA

TYR

12

LYS

THR

PHE

PRO

THR

GLU

PRO

LYS

13

ASP

LYS

ALA

ASP

GLU

THR

GLN

14

ALA

LEU

PRO

GLN

ARG

GLN

LYS

GLN

15

THR

VAL

THR

LEU

PRO

ALA

ASP

LEU

16

ASP

PHE

SER

LYS

GLN

LEU

GLN

SER

17

MET

SER

ALA

ASP

SER

THR

GLN

ALA

Samples:

sample_1: Nucleocapsid 251-419, [U-13C; U-15N; U-2H], 500 uM; sodium chloride 150 mM; HEPES 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - data analysis

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks