BMRB Entry 51682

Title:
Chemical_shift_FAM122A_29-120
Deposition date:
2022-11-01
Original release date:
2023-10-19
Authors:
Vos, Margaret; Page, Rebecca; Peti, Wolfgang
Citation:

Citation: Padi, Sathish; Vos, Margaret; Godek, Rachel; Fuller, James; Kruse, Thomas; Hein, Jamin; Nilsson, Jakob; Kelker, Matthew; Page, Rebecca; Peti, Wolfgang. "Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19"  Nature 625, 195-203 (2024).
PubMed: 38123684

Assembly members:

Assembly members:
entity_1, polymer, 94 residues, 10405 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHMT

Data sets:
Data typeCount
13C chemical shifts243
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FAM122A_29-1201

Entities:

Entity 1, FAM122A_29-120 94 residues - 10405 Da.

FAM122A residues 29-120; 92 residues starting from with GGG

1   HISMETGLYGLYGLYLEUARGARGSERASN
2   SERALAPROLEUILEHISGLYLEUSERASP
3   SERSERPROVALPHEGLNALAGLUALAPRO
4   SERALAARGARGASNSERTHRTHRPHEPRO
5   SERARGHISGLYLEULEULEUPROALASER
6   PROVALARGMETHISSERSERARGLEUHIS
7   GLNILELYSGLNGLUGLUGLYMETASPLEU
8   ILEASNARGGLUTHRVALHISGLUARGGLU
9   VALGLNTHRALAMETGLNILESERHISSER
10   TRPGLUGLUSER

Samples:

sample_1: FAM122A_29-120, [U-99% 15N], 400 uM; FAM122A_29-120, [U-99% 13C; U-99% 15N], 400 uM; Na2HPO4 20 mM; NaCl 150 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1.1 - collection, processing

CARA vCARA_1.9 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks