BMRB Entry 51674

Title:
DinB-PAD
Deposition date:
2022-10-28
Original release date:
2023-06-23
Authors:
Okeke, Damasus; Burmann, Bjorn
Citation:

Citation: Okeke, Damasus; Lidman, Jens; Matecko-Burmann, Irena; Burmann, Bjorn. "Thumb-domain dynamics modulate the functional repertoire of DNA-Polymerase IV (DinB)"  Nucleic Acids Res. 51, 7036-7052 (2023).
PubMed: 37260088

Assembly members:

Assembly members:
entity_1, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b-SUMO

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts118
1H chemical shifts627

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DinB-PAd1

Entities:

Entity 1, DinB-PAd 124 residues - Formula weight is not available

1   GLYILEASPGLUARGASPVALASNSERGLU
2   ARGLEUARGLYSSERVALGLYVALGLUARG
3   THRMETALAGLUASPILEHISHISTRPSER
4   GLUCYSGLUALAILEILEGLUARGLEUTYR
5   PROGLULEUGLUARGARGLEUALALYSVAL
6   LYSPROASPLEULEUILEALAARGGLNGLY
7   VALLYSLEULYSPHEASPASPPHEGLNGLN
8   THRTHRGLNGLUHISVALTRPPROARGLEU
9   ASNLYSALAASPLEUILEALATHRALAARG
10   LYSTHRTRPASPGLUARGARGGLYGLYARG
11   GLYVALARGLEUVALGLYLEUHISVALTHR
12   LEULEUASPPROGLNMETGLUARGGLNLEU
13   VALLEUGLYLEU

Samples:

sample_1: DinB-PAD, [U-100% 13C; U-100% 15N], 500 uM; KPi 50 mM; KCl 100 mM; Arginine 50 mM; Glutamine 50 mM; EDTA 1 mM; TCEP 1 mM; CHAPSO 0.1 mM; D2O 10%

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6 - collection

NMRPipe - processing

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks