BMRB Entry 51651

Title:
Rev7/Rev3-RBM1
Deposition date:
2022-10-04
Original release date:
2023-06-23
Authors:
Arianna, Gianluca; Geddes-Buehre, Dane; Korzhnev, Dmitry
Citation:

Citation: Arianna, Gianluca; Geddes-Buehre, Dane; Korzhnev, Dmitry. "Backbone and ILV side-chain methyl NMR resonance assignments of human Rev7/Rev3-RBM1 and Rev7/Rev3-RBM2 complexes"  Biomol. NMR Assign. 17, 107-114 (2023).
PubMed: 37129702

Assembly members:

Assembly members:
entity_1, polymer, 211 residues, Formula weight is not available
entity_2, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet-Duet-1

Data sets:
Data typeCount
13C chemical shifts772
15N chemical shifts208
1H chemical shifts484

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rev71
2Rev3-RBM12

Entities:

Entity 1, Rev7 211 residues - Formula weight is not available

1   METTHRTHRLEUTHRARGGLNASPLEUASN
2   PHEGLYGLNVALVALALAASPVALLEUCYS
3   GLUPHELEUGLUVALALAVALHISLEUILE
4   LEUTYRVALARGGLUVALTYRPROVALGLY
5   ILEPHEGLNLYSARGLYSLYSTYRASNVAL
6   PROVALGLNMETSERCYSHISPROGLULEU
7   ASNGLNTYRILEGLNASPTHRLEUHISCYS
8   VALLYSPROLEULEUGLULYSASNASPVAL
9   GLULYSVALVALVALVALILELEUASPLYS
10   GLUHISARGPROVALGLULYSPHEVALPHE
11   GLUILETHRGLNPROPROLEULEUSERILE
12   SERSERASPSERLEULEUSERHISVALGLU
13   GLNLEULEUALAALAPHEILELEULYSILE
14   SERVALCYSASPALAVALLEUASPHISASN
15   PROPROGLYCYSTHRPHETHRVALLEUVAL
16   HISTHRARGGLUALAALATHRARGASNMET
17   GLULYSILEGLNVALILELYSASPPHEPRO
18   TRPILELEUALAASPGLUGLNASPVALHIS
19   METHISASPPROARGLEUILEPROLEULYS
20   THRMETTHRSERASPILELEULYSMETGLN
21   LEUTYRVALGLUGLUARGALAHISLYSGLY
22   SER

Entity 2, Rev3-RBM1 27 residues - Formula weight is not available

1   METARGTHRALAASNILELEULYSPROLEU
2   METSERPROPROSERARGGLUGLUILEMET
3   ALATHRLEULEUASPHISASP

Samples:

sample_1: Rev7R124A, [U-15N; U-13C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.5 mM; Rev3-RBM1, [U-15N; U-13C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.5 mM

sample_2: Rev7K44A,R124A,A135D, [U-15N; U-12C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.75 mM; Rev3-RBM1, [U-15N; U-12C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.75 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HMCMCGCBCAsample_1isotropicsample_conditions_1
4D 13C-1H HMQC-NOESY-TROSYsample_2isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_1
13C-13C HMQC-NOESY-HMQCsample_2isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

SPARTA+ - chemical shift calculation

NMRPipe - processing

TALOS-N - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks