BMRB Entry 51647

Name: Backbone 1H, 13C and 15N chemical shift assignments of ubiquitin with a modified N-terminal twin-strep tag.
Published: 2023-03-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51647

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N chemical shift assignments of ubiquitin with a modified N-terminal twin-strep tag.

Deposition date:

2022-10-03

Original release date:

2023-03-23

Authors:

Nelson, Spencer; Deshmukh, Lalit

Citation:

Citation: Nelson, Spencer; Li, Yunan; Chen, Yue; Deshmukh, Lalit. "Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins" J. Am. Chem. Soc. 145, 7748-7752 (2023).
PubMed: 37010382

Assembly members:

Assembly members:
entity_1, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSWSHPQFERGGGSGGGSGG SSAWSHPQFERGSENLYFQG SGSMQIFVKTLTGKTITLEV EPSDTIENVKAKIQDKEGIP PDQQRLIFAGKQLEDGRTLS DYNIQKESTLHLVLRLRGG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	180
15N chemical shifts	91
1H chemical shifts	91

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ubiquitin with modified N-terminal twin-strep tag	1

Entities:

Entity 1, Ubiquitin with modified N-terminal twin-strep tag 119 residues - Formula weight is not available

1

GLY

SER

TRP

SER

HIS

PRO

GLN

PHE

GLU

ARG

2

GLY

SER

GLY

SER

GLY

3

SER

ALA

TRP

SER

HIS

PRO

GLN

PHE

GLU

4

ARG

GLY

SER

GLU

ASN

LEU

TYR

PHE

GLN

GLY

5

SER

GLY

SER

MET

GLN

ILE

PHE

VAL

LYS

THR

6

LEU

THR

GLY

LYS

THR

ILE

THR

LEU

GLU

VAL

7

GLU

PRO

SER

ASP

THR

ILE

GLU

ASN

VAL

LYS

8

ALA

LYS

ILE

GLN

ASP

LYS

GLU

GLY

ILE

PRO

9

PRO

ASP

GLN

ARG

LEU

ILE

PHE

ALA

GLY

10

LYS

GLN

LEU

GLU

ASP

GLY

ARG

THR

LEU

SER

11

ASP

TYR

ASN

ILE

GLN

LYS

GLU

SER

THR

LEU

12

HIS

LEU

VAL

LEU

ARG

LEU

ARG

GLY

Samples:

sample_1: Ubiquitin with modified N-terminal twin-strep tag, [U-100% 13C; U-100% 15N], 1 mM; D2O 7%; EDTA 1 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

CcpNMR - data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks