BMRB Entry 51634

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51634

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Title:
Main chain assignment of a RFFL fragment (26-145 aa).
Deposition date:
2022-09-20
Original release date:
2023-12-19
Authors:
Kumeta, Hiroyuki; Saio, Tomohide; Okiyoneda, Tsukasa
Citation:

Citation: Taniguchi, Sachiho; Ono, Yuji; Doi, Yukako; Taniguchi, Shogo; Matsuura, Yuta; Iwasaki, Ayuka; Hirata, Noriaki; Fukuda, Ryosuke; Inoue, Keitaro; Yamaguchi, Miho; Tashiro, Anju; Egami, Daichi; Aoki, Shunsuke; Kondoh, Yasumitsu; Honda, Kaori; Osada, Hiroyuki; Kumeta, Hiroyuki; Saio, Tomohide; Okiyoneda, Tsukasa. "Identification of alpha-Tocopherol succinate as an RFFL-substrate interaction inhibitor inducing peripheral CFTR stabilization and apoptosis"  Biochem. Pharmacol. 215, 115730-115730 (2023).
PubMed: 37543348

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis6-sumo-RFFL26-145

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQAYSNPGYSSFPSPTGLEP SCKSCGAHFANTARKQTCLD CKKNFCMTCSSQVGNGPRLC LLCQRFRATAFQREELMKMK VKDLRDYLSLHDISTEMCRE KEELVLLVLGQQPVISQEDR

Data sets:
Data typeCount
13C chemical shifts211
15N chemical shifts101
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RFFL26-1451
2ZN ion, 12
3ZN ion, 22

Entities:

Entity 1, RFFL26-145 120 residues - Formula weight is not available

1   METGLNALATYRSERASNPROGLYTYRSER
2   SERPHEPROSERPROTHRGLYLEUGLUPRO
3   SERCYSLYSSERCYSGLYALAHISPHEALA
4   ASNTHRALAARGLYSGLNTHRCYSLEUASP
5   CYSLYSLYSASNPHECYSMETTHRCYSSER
6   SERGLNVALGLYASNGLYPROARGLEUCYS
7   LEULEUCYSGLNARGPHEARGALATHRALA
8   PHEGLNARGGLUGLULEUMETLYSMETLYS
9   VALLYSASPLEUARGASPTYRLEUSERLEU
10   HISASPILESERTHRGLUMETCYSARGGLU
11   LYSGLUGLULEUVALLEULEUVALLEUGLY
12   GLNGLNPROVALILESERGLNGLUASPARG

Entity 2, ZN ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RFFL26-145, [U-99% 13C; U-99% 15N], 136 uM; D2O, [U-99% 2H], 5%; H2O 95%; DTT 1 mM; HEPES 50 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

VNMR v6.1C - collection

TOPSPIN v3.2 - collection, processing

NMRPipe - processing

SPARKY v3.113 - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III HD 600 MHz
  • Bruker AVANCE NEO 800 MHz
  • Agilent INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks