BMRB Entry 51520

Title:
bacteriophage lambda Ea22 C-terminal domain
Deposition date:
2022-07-19
Original release date:
2023-07-19
Authors:
Donaldson, Logan
Citation:

Citation: Tong, Jinge; Nejman-Falenczyk, BoZena; Bloch, Sylwia; Wegrzyn, Alicja; Wegrzyn, Grzegorz; Donaldson, Logan. "Ea22 Proteins from Lambda and Shiga Toxin-Producing Bacteriophages Balance Structural Diversity with Functional Similarity"  ACS Omega 5, 12236-12244 (2020).
PubMed: 32548406

Assembly members:

Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacteriophage sp.   Taxonomy ID: 38018   Superkingdom: Viruses   Kingdom: not available   Genus/species: lambda bacteriophage lambda bacteriophage

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pD441NH

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts64
1H chemical shifts439

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ea22, chain 11
2ea22, chain 21

Entities:

Entity 1, ea22, chain 1 93 residues - Formula weight is not available

6x-His tagged protein fragment. The last five amino acids IETAV are non-native.

1   HISHISHISHISHISHISMETSERASNGLU
2   VALARGGLUASPGLYASNGLNPHELEUVAL
3   VALARGHISPROGLYLYSTHRPROVALILE
4   LYSHISCYSTHRGLYASPLEUGLUGLUPHE
5   LEUARGGLNLEUILEGLUGLNASPPROLEU
6   VALTHRILEASPILEILETHRHISARGTYR
7   TYRGLYVALGLYGLYGLNTRPVALGLNASP
8   ALAGLYGLUTYRLEUHISMETMETSERASP
9   ALAGLYILEARGILELYSGLYGLUILEGLU
10   THRALAVAL

Samples:

sample_1: ea22, [U-99% 13C; U-99% 15N], 0.8 mM; TRIS 10 mM; sodium chloride 100 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.7; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 12C-filtered 13C-edited NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe v10.9 Rev 2021.051.13.12 - processing

ANALYSIS v2.52 - data analysis

CYANA v3.0 - structure solution

Rosetta v2021.16.61629 - refinement

NMR spectrometers:

  • Bruker AVANCE III 700 MHz
  • Varian INOVA 600 MHz

Related Database Links:

NCBI NP_040612.1
UNP P03756

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks