BMRB Entry 51518

Title:
CaBP1 C-Lobe Bound to CaV1.2 IQ-motif
Deposition date:
2022-07-19
Original release date:
2022-09-13
Authors:
Salveson, Ian; Ames, James
Citation:

Citation: Salveson, Ian; Ames, James. "Chemical shift assignments of the C-terminal domain of CaBP1 bound to the IQ-motif of voltage-gated Ca 2+ channel (Ca V 1.2)"  Biomol. NMR Assignments 16, 385-390 (2022).
PubMed: 36064846

Assembly members:

Assembly members:
entity_1, polymer, 70 residues, Formula weight is not available
entity_2, polymer, 18 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET 3a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts169
15N chemical shifts54
1H chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaBP1 C-lobe1
2IQ-motif2
3Calcium, 13
4Calcium, 23

Entities:

Entity 1, CaBP1 C-lobe 70 residues - Formula weight is not available

1   METILEGLYVALLYSGLULEUARGASPALA
2   PHEARGGLUPHEASPTHRASNGLYASPGLY
3   GLUILESERTHRSERGLULEUARGGLUALA
4   METARGLYSLEULEUGLYHISGLNVALGLY
5   HISARGASPILEGLUGLUILEILEARGASP
6   VALASPLEUASNGLYASPGLYARGVALASP
7   PHEGLUGLUPHEVALARGMETMETSERARG

Entity 2, IQ-motif 18 residues - Formula weight is not available

1   LYSPHETYRALATHRPHELEUILEGLNGLU
2   TYRPHEARGLYSPHELYSLYSARG

Entity 3, Calcium, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CaBP1 C-Lobe, [U-100% 15N], 0.5 mM; CaV1.2 IQ-motif 0.75 mM; Ca2+ 1 mM; Tris 10 mM

sample_2: CaBP1 C-Lobe, [U-100% 13C; U-100% 15N], 0.6 mM; CaV1.2 IQ-motif 0.9 mM; Ca2+ 1 mM; Tris 10 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1

Software:

NMRFx - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks