BMRB Entry 51506

Title:
Solid-state NMR 1H-13C-15N assignment of African cichlid nackednavirus capsid protein
Deposition date:
2022-06-29
Original release date:
2023-06-30
Authors:
Pfister, Sara; Meier, Beat; Wiegand, Thomas; Bockmann, Anja; Malar, Alexander
Citation:

Citation: Pfister, Sara; Rabl, Julius; Wiegand, Thomas; Mattei, Simone; Malar, Alexander; Lecoq, Lauriane; Seitz, Stefan; Bartenschlager, Ralf; Bockmann, Anja; Nassal, Michael; Boehringer, Daniel; Meier, Beat. "Structural conservation of HBV-like capsid proteins over hundreds of millions of years despite the shift from non-enveloped to enveloped life-style"  Nat. Commun. 14, 1574-1574 (2023).
PubMed: 36949039

Assembly members:

Assembly members:
entity_1, polymer, 175 residues, 3567274 Da.

Natural source:

Natural source:   Common Name: bony fishes   Taxonomy ID: 27755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ophthalmotilapia ventralis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSF_T7-CNDVcOpt

Data sets:
Data typeCount
13C chemical shifts443
15N chemical shifts108
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1African cichlid nackednavirus capsid1

Entities:

Entity 1, African cichlid nackednavirus capsid 175 residues - 3567274 Da.

1   METGLYTHRPHEILEGLULEUVALLYSASN
2   METLYSGLYTYRLYSGLULEULEULEUPRO
3   METGLUMETVALPROLEUPROALAVALVAL
4   LEULYSHISVALLYSLEUILELEUTHRSER
5   GLNLYSGLUHISGLNPROTRPMETTHRGLU
6   METALALEULYSALAASPGLNCYSLEUILE
7   HISLYSALATHRLEUASPLEUALAGLYLYS
8   ALATHRSERASNGLUALALYSPROLEUILE
9   GLUALAMETGLNGLNILEILELEUALAMET
10   THRARGGLULEUTRPGLYGLNILEGLNARG
11   HISHISTYRGLYILEVALGLNVALGLUHIS
12   TYRVALLYSGLNILETHRLEUTRPGLNASP
13   THRPROGLNALAPHEARGGLYASPGLNPRO
14   LYSPROPROSERPHEARGSERASPGLYPRO
15   THRARGGLYGLNGLYSERPHEARGPROPHE
16   PHEARGGLYARGGLYARGGLYARGGLYARG
17   GLYARGGLYSERGLNSERPROALAARGLYS
18   GLYPROLEUPROLYS

Samples:

sample_1: African cichlid nackednavirus capsid protein, [U-100% 13C; U-100% 15N], n.d. mg/L; HEPES 50 mM; DTT 5 mM; DSS n.d. uM; sodium azide n.d. uM

sample_2: African cichlid nackednavirus capsid protein, [U-100% 13C; U-100% 15N], n.d. mg/L; DTT 5 mM; DSS n.d. uM; sodium azide n.d. uM; TRIS 50 mM; EDTA 5 mM; sodium chloride 50 mM

sample_3: African cichlid nackednavirus capsid protein, [U-100% 13C; U-100% 15N], n.d. mg/L; DTT 5 mM; DSS n.d. uM; sodium azide n.d. uM; TRIS 50 mM; EDTA 5 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7.5; temperature: 277.15 K

sample_conditions_2: pH: 7.5; temperature: 294 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1isotropicsample_conditions_1
NCAsample_3isotropicsample_conditions_1
NCOsample_3isotropicsample_conditions_1
NCACBsample_1isotropicsample_conditions_1
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_1
CCCsample_3isotropicsample_conditions_1
CANcoCAsample_3isotropicsample_conditions_1
NcoCACBsample_3isotropicsample_conditions_1
hNHsample_2isotropicsample_conditions_2
hCHsample_2isotropicsample_conditions_2
hCANHsample_2isotropicsample_conditions_2
hNCAHsample_2isotropicsample_conditions_2
hCAcoNHsample_2isotropicsample_conditions_2
hNcoCAHsample_2isotropicsample_conditions_2

Software:

TOPSPIN v3.5 - collection, processing

CcpNMR v2 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks