BMRB Entry 51433
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51433
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Porter, Lauren; Kim, Allen; Rimal, Swechha; Looger, Loren; Majumdar, Ananya; Mensh, Brett; Starich, Mary; Strub, Marie-Paule. "Many dissimilar NusG protein domains switch between alpha-helix and beta-sheet folds" Nat. Commun. 13, 3802-3802 (2022).
PubMed: 35778397
Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available
Natural source: Common Name: Candidatus Kryptobacter tengchongensis Taxonomy ID: 1643429 Superkingdom: Bacteria Kingdom: not available Genus/species: Candidatus Kryptobacter tengchongensis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPAL7
Entity Sequences (FASTA):
entity_1: AELERIDVPFRVGDSVKVID
GPFTDFSGVVQEVNSEKMKL
KVMINIFGRKTPVELDFTQV
EIEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 163 |
| 15N chemical shifts | 54 |
| 1H chemical shifts | 54 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Variant 8 CTD | 1 |
Entities:
Entity 1, Variant 8 CTD 64 residues - Formula weight is not available
| 1 | ALA | GLU | LEU | GLU | ARG | ILE | ASP | VAL | PRO | PHE | ||||
| 2 | ARG | VAL | GLY | ASP | SER | VAL | LYS | VAL | ILE | ASP | ||||
| 3 | GLY | PRO | PHE | THR | ASP | PHE | SER | GLY | VAL | VAL | ||||
| 4 | GLN | GLU | VAL | ASN | SER | GLU | LYS | MET | LYS | LEU | ||||
| 5 | LYS | VAL | MET | ILE | ASN | ILE | PHE | GLY | ARG | LYS | ||||
| 6 | THR | PRO | VAL | GLU | LEU | ASP | PHE | THR | GLN | VAL | ||||
| 7 | GLU | ILE | GLU | LYS |
Samples:
sample_1: Variant 8 CTD, [U-100% 13C; U-100% 15N], 80 uM; potassium phosphate 100 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMRPipe - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks