BMRB Entry 51429

Title:
Full-length Variant 5 (CTD only)
Deposition date:
2022-05-06
Original release date:
2022-05-16
Authors:
Porter, Lauren; Starich, Mary
Citation:

Citation: Porter, Lauren; Kim, Allen; Rimal, Swechha; Looger, Loren; Majumdar, Ananya; Mensh, Brett; Starich, Mary; Strub, Marie-Paule. "Many dissimilar NusG protein domains switch between alpha-helix and beta-sheet folds"  Nat. Commun. 13, 3802-3802 (2022).
PubMed: 35778397

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Calditerrivibrio nitroreducens   Taxonomy ID: 477976   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Calditerrivibrio nitroreducens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPAL7

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts128
15N chemical shifts45
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Variant 5 full-length (CTD only)1

Entities:

Entity 1, Variant 5 full-length (CTD only) 64 residues - Formula weight is not available

1   PHEILEASPTHRLYSSERGLUGLUPHELYS
2   LYSGLYASPTHRILELEUILELYSASPGLY
3   PROPHELYSASPPHEVALGLYILEPHEGLN
4   GLUGLULEUASPSERLYSGLYARGVALSER
5   ILELEULEULYSTHRLEUALALEUGLNPRO
6   ARGILETHRVALASPLYSASPMETILEGLU
7   LYSLEUHISASN

Samples:

sample_1: Variant 5 full-length (CTD only), [U-13C; U-15N; U-2H], 135 uM; HEPES 25 mM; NaCl 50 mM; deuterated glycerol (Sigma Aldrich) 5%; DTT 1 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks