BMRB Entry 51402

Title:
Bacillus subtilis SepF protein assembly (wild type)
Deposition date:
2022-04-19
Original release date:
2022-07-11
Authors:
Liu, Wenjing
Citation:

Citation: Zhang, Chang; Liu, Wenjing; Deng, Jing; Ma, Shaojie; Chang, Ziwei; Yang, Jun. "Structural Insights into the Interaction between Bacillus subtilis SepF Assembly and FtsZ by Solid-State NMR Spectroscopy"  J. Phys. Chem. B 126, 5219-5230 (2022).
PubMed: 35799411

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SepF monomer1

Entities:

Entity 1, SepF monomer 151 residues - Formula weight is not available

1   METSERMETLYSASNLYSLEULYSASNPHE
2   PHESERMETGLUASPGLUGLUTYRGLUTYR
3   GLUTYRILEGLUTHRGLUARGGLUSERHIS
4   GLUGLUHISGLUGLNLYSGLULYSPROALA
5   TYRASNGLYASNLYSPROALAGLYLYSGLN
6   ASNVALVALSERLEUGLNSERVALGLNLYS
7   SERSERLYSVALVALLEUSERGLUPROARG
8   VALTYRALAGLUALAGLNGLUILEALAASP
9   HISLEULYSASNARGARGALAVALVALVAL
10   ASNLEUGLNARGILEGLNHISASPGLNALA
11   LYSARGILEVALASPPHELEUSERGLYTHR
12   VALTYRALAILEGLYGLYASPILEGLNARG
13   ILEGLYSERASPILEPHELEUCYSTHRPRO
14   ASPASNVALASPVALSERGLYTHRILESER
15   GLULEUILESERGLUASPGLUHISGLNARG
16   TRP

Samples:

sample_1: SepF 57-151, [U-100% 13C; U-100% 15N], 38.9 % w/w

sample_conditions_1: pH: 8.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-detected NCAsample_1isotropicsample_conditions_1
2D 13C-13C DARRsample_1isotropicsample_conditions_1
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CONCAsample_1isotropicsample_conditions_1
2D NcaCXsample_1isotropicsample_conditions_1

Software:

VNMR - collection

NMRPipe - data analysis

SPARKY - chemical shift assignment

NMR spectrometers:

  • Varian Uniform NMR System 600 MHz