BMRB Entry 51401

Title:
SNAC-mEpoR-TM
Deposition date:
2022-04-17
Original release date:
2022-06-13
Authors:
Mravic, Marco
Citation:

Citation: Mravic, Marco. "Spectra of mEpoR transmembrane fragment in 2H-DPC, monomeric state"  .

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SHHWNDPLILTLSLILVLIS LLLTVLALLSHRR

Data sets:
Data typeCount
13C chemical shifts117
15N chemical shifts31
1H chemical shifts241

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mEpoR-TM_monomer1

Entities:

Entity 1, mEpoR-TM_monomer 33 residues - Formula weight is not available

1   SERHISHISTRPASNASPPROLEUILELEU
2   THRLEUSERLEUILELEUVALLEUILESER
3   LEULEULEUTHRVALLEUALALEULEUSER
4   HISARGARG

Samples:

sample_1: mEpoR TM, [U-95% 13C; U-95% 15N], 1 mM; D2O 5%; H2O 95%; sodium chloride 20 mM; EDTA 1 mM; sodium acetate, [U-2H], 50 mM; DPC, [U-99% 2H], 400 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 5.2; pressure: 1 atm; temperature: 318.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

TOPSPIN v4.0.2 - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P14753
AlphaFold Q63852

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks