BMRB Entry 51391

Title:
NMR Evidence for the Conformational Change of Phage Protein gVp Upon Binding to ssDNA
Deposition date:
2022-04-07
Original release date:
2022-07-05
Authors:
Kedem, Smadar; Hassid, Roni Rene; Shamir, Yoav; Goldbourt, Amir
Citation:

Citation: Kedem, Smadar; Hassid, Roni Rene; Shamir, Yoav; Goldbourt, Amir. "Conformational Changes in Ff Phage Protein gVp upon Complexation with Its Viral Single-Stranded DNA Revealed Using Magic-Angle Spinning Solid-State NMR"  Viruses 14, 1264-1264 (2022).
PubMed: 35746735

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts405
15N chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gVp1

Entities:

Entity 1, gVp 87 residues - Formula weight is not available

1   METILELYSVALGLUILELYSPROSERGLN
2   ALAGLNPHETHRTHRARGSERGLYVALSER
3   ARGGLNGLYLYSPROTYRSERLEUASNGLU
4   GLNLEUCYSTYRVALASPLEUGLYASNGLU
5   TYRPROVALLEUVALLYSILETHRLEUASP
6   GLUGLYGLNPROALATYRALAPROGLYLEU
7   TYRTHRVALHISLEUSERSERPHELYSVAL
8   GLYGLNPHEGLYSERLEUMETILEASPARG
9   LEUARGLEUVALPROALALYS

Samples:

sample_1: gVp from fd bacteriophage, [U-100% 13C; U-100% 15N], 200 ± 50 g/L; NaCl 200 mM; EDTA 1 mM; Tris-HCl 10 mM

sample_2: gVp from fd bacteriophage, [1,3-13C]-glycerol, 200 ± 50 g/L; NaCl 200 mM; EDTA 1 mM; Tris-HCl 10 mM

sample_conditions_1: pH: 7.4; temperature: 263 K

Experiments:

NameSampleSample stateSample conditions
2D DARR5sample_1isotropicsample_conditions_1
2D DARR15sample_1isotropicsample_conditions_1
2D DARR100sample_1isotropicsample_conditions_1
2D DARR250sample_1isotropicsample_conditions_1
2D RFDR6sample_1isotropicsample_conditions_1
2D INADEQUATEsample_1isotropicsample_conditions_1
3D NCOCX25sample_1isotropicsample_conditions_1
3D NCACX25sample_1isotropicsample_conditions_1
2D DARR5sample_2isotropicsample_conditions_1
2D DARR15sample_2isotropicsample_conditions_1
2D DARR100sample_2isotropicsample_conditions_1
2D DARR300sample_2isotropicsample_conditions_1
2D NCAsample_2isotropicsample_conditions_1
2D NCOsample_2isotropicsample_conditions_1
3D NCOCX100sample_2isotropicsample_conditions_1
3D NCACX100sample_2isotropicsample_conditions_1
2D CORD150sample_2isotropicsample_conditions_1
2D CORD300sample_2isotropicsample_conditions_1

Software:

NMRPipe vv9.3 - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz