BMRB Entry 51390

Title:
HN-S CTD
Deposition date:
2022-04-06
Original release date:
2022-05-04
Authors:
Price, Caitlin; Hussain, Alya; Henen, Morkos; Vogeli, Beat; Sette, Marco; Barbato, Gaetano; Boelens, Rolf
Citation:

Citation: Price, Caitlin; Hussain, Alya; Henen, Morkos; Vogeli, Beat; Sette, Marco; Barbato, Gaetano; Boelens, Rolf. "Histone-like nucleoid structuring protein interaction with DNA in Shigella flexneri"  .

Assembly members:

Assembly members:
entity_1, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Shigella flexneri   Taxonomy ID: 623   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shigella flexneri

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet28(a)+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RAQRPAKYSYVDENGETKTW TGQGRTPAVIKKAMDEQGKS LDDFLIKQ

Data sets:
Data typeCount
13C chemical shifts130
15N chemical shifts41
1H chemical shifts216

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HN-S CTD1

Entities:

Entity 1, HN-S CTD 48 residues - Formula weight is not available

1   ARGALAGLNARGPROALALYSTYRSERTYR
2   VALASPGLUASNGLYGLUTHRLYSTHRTRP
3   THRGLYGLNGLYARGTHRPROALAVALILE
4   LYSLYSALAMETASPGLUGLNGLYLYSSER
5   LEUASPASPPHELEUILELYSGLN

Samples:

sample_1: HN-S CTD, [U-100% 13C; U-100% 15N], 700 uM; NaCl 20 mM

sample_conditions_1: ionic strength: 30 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment, peak picking

TOPSPIN v4.1.1 - collection, data analysis

VNMR - collection

NMRDraw - data analysis, peak picking, processing

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz
  • Varian INOVA 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks