BMRB Entry 51380

Name: 1H, 13C and 15N Backbone Chemical Shift Assignments of GTP-bound Rab3a
Published: 2022-07-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51380

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Backbone Chemical Shift Assignments of GTP-bound Rab3a

Deposition date:

2022-03-23

Original release date:

2022-07-12

Authors:

Lv, Guohua; Ko, Myung Soo; Das, Tapojyoti; Eliezer, David

Citation:

Citation: Lv, Guohua; Ko, Myung Soo; Das, Tapojyoti; Eliezer, David. "Molecular and Functional Interactions of Alpha-Synuclein with Rab3a" J. Biol. Chem. 298, 102239-102239 (2022).
PubMed: 35809645

Assembly members:

Assembly members:
entity_1, polymer, 172 residues, Formula weight is not available
entity_GTP, non-polymer, 523.180 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SDQNFDYMFKILIIGNSSVG KTSFLFRYADDSFTPAFVST VGIDFKVKTIYRNDKRIKLQ IWDTAGLERYRTITTAYYRG AMGFILMYDITNEESFNAVQ DWSTQIKTYSWDNAQVLLVG NKADMEDERVVSSERGRQLA DHLGFEFFEASAKDNINVKQ TFERLVDVIAEK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	496
15N chemical shifts	164
1H chemical shifts	164

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rab3a_15-186	1
2	GTP	2

Entities:

Entity 1, Rab3a_15-186 172 residues - Formula weight is not available

1

SER

ASP

GLN

ASN

PHE

ASP

TYR

MET

PHE

LYS

2

ILE

LEU

ILE

GLY

ASN

SER

VAL

GLY

3

LYS

THR

SER

PHE

LEU

PHE

ARG

TYR

ALA

ASP

4

ASP

SER

PHE

THR

PRO

ALA

PHE

VAL

SER

THR

5

VAL

GLY

ILE

ASP

PHE

LYS

VAL

LYS

THR

ILE

6

TYR

ARG

ASN

ASP

LYS

ARG

ILE

LYS

LEU

GLN

7

ILE

TRP

ASP

THR

ALA

GLY

LEU

GLU

ARG

TYR

8

ARG

THR

ILE

THR

ALA

TYR

ARG

GLY

9

ALA

MET

GLY

PHE

ILE

LEU

MET

TYR

ASP

ILE

10

THR

ASN

GLU

SER

PHE

ASN

ALA

VAL

GLN

11

ASP

TRP

SER

THR

GLN

ILE

LYS

THR

TYR

SER

12

TRP

ASP

ASN

ALA

GLN

VAL

LEU

VAL

GLY

13

ASN

LYS

ALA

ASP

MET

GLU

ASP

GLU

ARG

VAL

14

VAL

SER

GLU

ARG

GLY

ARG

GLN

LEU

ALA

15

ASP

HIS

LEU

GLY

PHE

GLU

PHE

GLU

ALA

16

SER

ALA

LYS

ASP

ASN

ILE

ASN

VAL

LYS

GLN

17

THR

PHE

GLU

ARG

LEU

VAL

ASP

VAL

ILE

ALA

18

GLU

LYS

Entity 2, GTP - C10 H16 N5 O14 P3 - 523.180 Da.

1

GTP

Samples:

sample_1: Rab3a_15-186, [U-13C; U-15N; U-2H], 260 uM; GTP 1 mM; Tris 50 mM; NaCl 150 mM; DTT 2 mM; MgCl2 10 mM; NaN3 0.05%

sample_conditions_1: pH: 7.1; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment, peak picking

TOPSPIN - collection

NMRPipe - processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz
Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks