BMRB Entry 51374

Title:
15N-1H chemical shifts for the murine Syk tandem SH2 domains flexible linker construct [tSH2-FX, Ser 8 to Gln 264 with interdomain residues 119-162 replaced by a 20-amino-acid flexible linker (GGS)3GS(GGS)3] complexed with N-IHP peptide
Deposition date:
2022-03-18
Original release date:
2022-04-18
Authors:
Feng, Chao; Post, Carol
Citation:

Citation: Feng, Chao; Post, Carol Beth. "Insights into the allosteric regulation of Syk association with receptor ITAM, a multi-state equilibrium."  Phys. Chem. Chem. Phys. 18, 5807-5818 (2016).
PubMed: 26468009

Assembly members:

Assembly members:
entity_1, polymer, 233 residues, Formula weight is not available
entity_2, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30a(+)

Data sets:
Data typeCount
15N chemical shifts121
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1murine Syk tandem SH2 domains flexible linker construct1
2N-IHP peptide2

Entities:

Entity 1, murine Syk tandem SH2 domains flexible linker construct 233 residues - Formula weight is not available

1   SERALAASNHISLEUTHRTYRPHEPHEGLY
2   ASNILETHRARGGLUGLUALAGLUASPTYR
3   LEUVALGLNGLYGLYMETTHRASPGLYLEU
4   TYRLEULEUARGGLNSERARGASNTYRLEU
5   GLYGLYPHEALALEUSERVALALAHISASN
6   ARGLYSALAHISHISTYRTHRILEGLUARG
7   GLULEUASNGLYTHRTYRALAILESERGLY
8   GLYARGALAHISALASERPROALAASPLEU
9   CYSHISTYRHISSERGLNGLUPROASPGLY
10   LEUILECYSLEULEULYSLYSPROPHEASN
11   ARGPROPROGLYVALGLNPROLYSTHRGLY
12   PROGLYGLYSERGLYGLYSERGLYGLYSER
13   GLYSERGLYGLYSERGLYGLYSERGLYGLY
14   SERGLULYSMETPROTRPPHEHISGLYASN
15   ILESERARGASPGLUSERGLUGLNTHRVAL
16   LEUILEGLYSERLYSTHRASNGLYLYSPHE
17   LEUILEARGALAARGASPASNSERGLYSER
18   TYRALALEUCYSLEULEUHISGLUGLYLYS
19   VALLEUHISTYRARGILEASPARGASPLYS
20   THRGLYLYSLEUSERILEPROGLUGLYLYS
21   LYSPHEASPTHRLEUTRPGLNLEUVALGLU
22   HISTYRSERTYRLYSPROASPGLYLEULEU
23   ARGVALLEUTHRVALPROCYSGLNLYSILE
24   GLYALAGLN

Entity 2, N-IHP peptide 11 residues - Formula weight is not available

1   ACEPROASPPTRGLUPROILEARGLYSGLY
2   NH2

Samples:

sample_1: murine Syk tandem SH2 domains flexible linker construct, [U-99% 15N], 0.3 mM; sodium phosphate 50 mM; DTT 5 mM; sodium azide 0.02%; N-IHP peptide 6 mM

sample_conditions_1: ionic strength: 0.13 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks