BMRB Entry 51366

Title:
15N-1H chemical shifts for the unphosphorylated murine Syk tandem SH2 domains (Ser 8 to Gln 264) complexed with N-IHP peptide
Deposition date:
2022-03-17
Original release date:
2022-04-18
Authors:
Feng, Chao; Post, Carol
Citation:

Citation: Feng, Chao; Post, Carol Beth. "Insights into the allosteric regulation of Syk association with receptor ITAM, a multi-state equilibrium."  Phys. Chem. Chem. Phys. 18, 5807-5818 (2016).
PubMed: 26468009

Assembly members:

Assembly members:
entity_1, polymer, 257 residues, Formula weight is not available
entity_2, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30a(+)

Data sets:
Data typeCount
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unphosphorylated murine Syk tandem SH2 domains (Ser 8 to Gln 264)1
2N-IHP peptide2

Entities:

Entity 1, unphosphorylated murine Syk tandem SH2 domains (Ser 8 to Gln 264) 257 residues - Formula weight is not available

1   SERALAASNHISLEUTHRTYRPHEPHEGLY
2   ASNILETHRARGGLUGLUALAGLUASPTYR
3   LEUVALGLNGLYGLYMETTHRASPGLYLEU
4   TYRLEULEUARGGLNSERARGASNTYRLEU
5   GLYGLYPHEALALEUSERVALALAHISASN
6   ARGLYSALAHISHISTYRTHRILEGLUARG
7   GLULEUASNGLYTHRTYRALAILESERGLY
8   GLYARGALAHISALASERPROALAASPLEU
9   CYSHISTYRHISSERGLNGLUPROASPGLY
10   LEUILECYSLEULEULYSLYSPROPHEASN
11   ARGPROPROGLYVALGLNPROLYSTHRGLY
12   PROPHEGLUASPLEULYSGLUASNLEUILE
13   ARGGLUTYRVALLYSGLNTHRTRPASNLEU
14   GLNGLYGLNALALEUGLUGLNALAILEILE
15   SERGLNLYSPROGLNLEUGLULYSLEUILE
16   ALATHRTHRALAHISGLULYSMETPROTRP
17   PHEHISGLYASNILESERARGASPGLUSER
18   GLUGLNTHRVALLEUILEGLYSERLYSTHR
19   ASNGLYLYSPHELEUILEARGALAARGASP
20   ASNSERGLYSERTYRALALEUCYSLEULEU
21   HISGLUGLYLYSVALLEUHISTYRARGILE
22   ASPARGASPLYSTHRGLYLYSLEUSERILE
23   PROGLUGLYLYSLYSPHEASPTHRLEUTRP
24   GLNLEUVALGLUHISTYRSERTYRLYSPRO
25   ASPGLYLEULEUARGVALLEUTHRVALPRO
26   CYSGLNLYSILEGLYALAGLN

Entity 2, N-IHP peptide 11 residues - Formula weight is not available

1   ACEPROASPPTRGLUPROILEARGLYSGLY
2   NH2

Samples:

sample_1: unphosphorylated murine Syk tandem SH2 domains (Ser 8 to Gln 264), [U-99% 15N], 0.3 mM; sodium phosphate 50 mM; DTT 5 mM; sodium azide 0.02%; N-IHP peptide 6 mM

sample_conditions_1: ionic strength: 0.13 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks