BMRB Entry 51314

Title:
human FUS 454-526
Deposition date:
2022-02-08
Original release date:
2022-03-28
Authors:
Lenard, Aneta; Bourgeois, Benjamin; Zhou, Qishun; Madl, Tobias
Citation:

Citation: Lenard, Aneta; Zhou, Qishun; Madreiter-Sokolowski, Corina; Bourgeois, Benjamin; Habacher, Hermann; Khanna, Yukti; Madl, Tobias. "EGCG Promotes FUS Condensate Formation in a Methylation-Dependent Manner"  Cells 11, 592-592 (2022).
PubMed: 35203243

Assembly members:

Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts115
15N chemical shifts67
1H chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human FUS 454-5261

Entities:

Entity 1, human FUS 454-526 73 residues - Formula weight is not available

1   GLYPROGLYGLYGLYPROGLYGLYSERHIS
2   METGLYGLYASNTYRGLYASPASPARGARG
3   GLYGLYARGGLYGLYTYRASPARGGLYGLY
4   TYRARGGLYARGGLYGLYASPARGGLYGLY
5   PHEARGGLYGLYARGGLYGLYGLYASPARG
6   GLYGLYPHEGLYPROGLYLYSMETASPSER
7   ARGGLYGLUHISARGGLNASPARGARGGLU
8   ARGPROTYR

Samples:

sample_1: Fused-In-Sarcoma, [U-100% 13C; U-100% 15N], 583 uM; succinic acid 50 mM; sodium chloride 150 mM; TCEP 2 mM; sodium azide 0.04%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
HNCANNHsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4 - chemical shift assignment, data analysis, peak picking, processing

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks