BMRB Entry 51308

Title:
1H15N HSQC Chemical Shifts for K66A MIF
Deposition date:
2022-02-06
Original release date:
2022-05-27
Authors:
Skeens, Erin; Lisi, George
Citation:

Citation: Skeens, Erin; Gadzuk-Shea, Meagan; Shah, Dilip; Bhandari, Vineet; Schweppe, Devin; Berlow, Rebecca; Lisi, George. "Redox-dependent structure and dynamics of macrophage migration inhibitory factor reveal sites of latent allostery"  Structure 30, 840-850 (2022).
PubMed: 35381187

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11b

Data sets:
Data typeCount
15N chemical shifts90
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K66A MIF monomer, 11
2K66A MIF monomer, 21
3K66A MIF monomer, 31

Entities:

Entity 1, K66A MIF monomer, 1 114 residues - Formula weight is not available

1   PROMETPHEILEVALASNTHRASNVALPRO
2   ARGALASERVALPROASPGLYPHELEUSER
3   GLULEUTHRGLNGLNLEUALAGLNALATHR
4   GLYLYSPROPROGLNTYRILEALAVALHIS
5   VALVALPROASPGLNLEUMETALAPHEGLY
6   GLYSERSERGLUPROCYSALALEUCYSSER
7   LEUHISSERILEGLYALAILEGLYGLYALA
8   GLNASNARGSERTYRSERLYSLEULEUCYS
9   GLYLEULEUALAGLUARGLEUARGILESER
10   PROASPARGVALTYRILEASNTYRTYRASP
11   METASNALAALAASNVALGLYTRPASNASN
12   SERTHRPHEALA

Samples:

sample_1: WT MIF, [U-99% 15N], 1.0 mM; sodium phosphate 20 mM; D2O 10%; EDTA 1 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - data analysis

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks