BMRB Entry 51277

Title:
Chemical shift assignment of LLL myristoylated Hisactophilin at pH 6.2
Deposition date:
2022-01-13
Original release date:
2022-06-24
Authors:
MacKenzie, Duncan; Leo, Christopher; Ko, Travis; Shah, Purnak; Ney, Mikaela; Smith, Martin; Meiering, Elizabeth
Citation:

Citation: MacKenzie, Duncan; Schaefer, Anna; Steckner, Julia; Leo, Christopher; Naser, Dalia; Artikis, Eforsini; Broom, Aron; Ko, Travis; Shah, Purnak; Ney, Mikaela; Tran, Elisa; Smith, Martin; Fuglestad, Brian; Wand, A Josh; Brooks, Charles; Meiering, Elizabeth. "A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein"  Proc. Natl. Acad. Sci. U.S.A. 119, e2119686119-e2119686119 (2022).
PubMed: 35737838

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cellular slime molds   Taxonomy ID: 44689   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
Data typeCount
15N chemical shifts92
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LLL myristoylated hisactophilin1

Entities:

Entity 1, LLL myristoylated hisactophilin 118 residues - Formula weight is not available

1   METGLYASNARGALALEULYSSERHISHIS
2   GLYHISPHELEUSERALAGLUGLYGLUALA
3   VALLYSTHRHISHISGLYHISHISASPHIS
4   HISTHRHISPHEHISVALGLUASNHISGLY
5   GLYLYSVALALALEULYSTHRHISCYSGLY
6   LYSTYRLEUSERILEGLYASPHISLYSGLN
7   VALTYRLEUSERHISHISLEUHISGLYASP
8   HISSERLEUPHEHISLEUGLUHISHISGLY
9   GLYLYSVALSERLEULYSGLYHISHISHIS
10   HISTYRLEUSERALAASPHISHISGLYHIS
11   VALSERTHRLYSGLUHISHISASPHISASP
12   THRTHRPHEGLUGLUILEILEILE

Samples:

sample_1: MES 50 mM; EDTA 1 mM; DTT 1 mM; DSS 1 mM; LLL myristoylated hisactophilin, [U-99% 15N], 28 mg/mL

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 299.25 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - chemical shift assignment, collection, processing

xwinnmr - collection, processing

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks