BMRB Entry 51258

Title:
Backbone and sidechain assignments of Actophorin protein from Entamoeba histolytica
Deposition date:
2022-01-05
Original release date:
2022-01-14
Authors:
Aggarwal, Priyanka; Kumar, Nitesh; Samudrala, Gourinath; Bhavesh, Neel
Citation:

Citation: Kumar, Nitesh; Rath, Pragyan Parimita; Aggarwal, Priyanka; Maiti, Sankar; Bhavesh, Neel Sarovar; Gourinath, Samudrala. "Unravelling the Biology of EhActo as the First Cofilin From Entamoeba histolytica"  Front. Cell Dev. Biol. 10, 785680-785680 (2022).
PubMed: 35281106

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Entamoeba histolytica   Taxonomy ID: 5759   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Entamoeba histolytica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Data sets:
Data typeCount
13C chemical shifts534
15N chemical shifts130
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Actophorin1

Entities:

Entity 1, Actophorin 146 residues - Formula weight is not available

1   METALAMETALAGLYILEGLNLEUALAASP
2   GLUVALTHRSERVALTYRASNASPPHELYS
3   LEUSERHISLYSTYRARGTYRILEVALPHE
4   LYSMETASNASPGLYMETTHRGLUVALVAL
5   VALGLULYSTHRALAGLULYSASNALATHR
6   TYRASPASPPHELEULYSASPLEUPROGLU
7   LYSSERALAARGTYRALAVALTYRASPLEU
8   GLUTYRASPTHRPROGLUGLYLEUARGGLN
9   LYSILEILEPHETYRLEUTRPTHRPROGLU
10   GLYCYSLYSILEARGGLULYSMETLEUTYR
11   SERALATHRLYSALATHRILELYSGLNALA
12   LEUVALGLYLEUSERALAGLUILEGLNALA
13   THRASPALAGLYGLULEUASNLEUASPGLU
14   VALILEALALYSVALLYSTHRILELEUGLU
15   HISHISHISHISHISHIS

Samples:

sample_1: Actophorin, [U-99% 13C; U-99% 15N], 1 ± 0.01 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; NaCl 60 mM; KCl 2.7 mM

sample_conditions_1: ionic strength: 60 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5pl6 - collection, processing

CARA - chemical shift assignment, data analysis

CS-Rosetta - structure solution

NMR spectrometers:

  • Bruker AVANCE III 500.15 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks