BMRB Entry 51222

Title:
Calmodulin bound to the beta-subunit of the retinal cyclic nucleotide-gated cation channel
Deposition date:
2021-12-06
Original release date:
2022-02-18
Authors:
Bej, Aritra; Ames, James
Citation:

Citation: Bej, Aritra; Ames, James. "Chemical shift assignments of calmodulin bound to the beta-subunit of a retinal cyclic nucleotide-gated channel (CNGB1)"  Biomol. NMR Assignments 16, 147-151 (2022).
PubMed: 35107779

Assembly members:

Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11b

Data sets:
Data typeCount
13C chemical shifts459
15N chemical shifts122
1H chemical shifts704

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin1
2CA ion, 12
3CA ion, 22
4CA ion, 32
5CA ion, 42

Entities:

Entity 1, Calmodulin - Formula weight is not available

1   METALAASPGLNLEUTHRGLUGLUGLNILE
2   ALAGLUPHELYSGLUALAPHESERLEUPHE
3   ASPLYSASPGLYASPGLYTHRILETHRTHR
4   LYSGLULEUGLYTHRVALMETARGSERLEU
5   GLYGLNASNPROTHRGLUALAGLULEUGLN
6   ASPMETILEASNGLUVALASPALAASPGLY
7   ASNGLYTHRILEASPPHEPROGLUPHELEU
8   THRMETMETALAARGLYSMETLYSASPTHR
9   ASPSERGLUGLUGLUILEARGGLUALAPHE
10   ARGVALPHEASPLYSASPGLYASNGLYTYR
11   ILESERALAALAGLULEUARGHISVALMET
12   THRASNLEUGLYGLULYSLEUTHRASPGLU
13   GLUVALASPGLUMETILEARGGLUALAASP
14   ILEASPGLYASPGLYGLNVALASNTYRGLU
15   GLUPHEVALGLNMETMETTHRALALYS

Entity 2, CA ion, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calmodulin, [U-99% 13C; U-99% 15N], 0.5 mM; Tris-d11 20 mM; CaCl2 1 mM

sample_2: Calmodulin, [U-99% 13C; U-99% 15N], 0.5 mM; Tris-d11 20 mM; CaCl2 1 mM

sample_3: Calmodulin, [U-10% 13C; U-99% 15N], 0.5 mM; Tris-d11 20 mM; CaCl2 1 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 13C-separated NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1

Software:

NMRPipe - processing

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 800 MHz

Related Database Links:

NCBI AAD45181

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks