BMRB Entry 51205

Title:
Musashi-1 C terminal deltaSeq1
Deposition date:
2021-12-02
Original release date:
2022-05-11
Authors:
Huang, Jie-rong
Citation:

Citation: Chiu, Shih-Hui; Ho, Wen-Lin; Sun, Yung-Chen; Kuo, Jean-Cheng; Huang, Jie-Rong. "Phase separation driven by interchangeable properties in the intrinsically disordered regions of protein paralogs"  Commun. Biol. 5, 400-400 (2022).
PubMed: 35487971

Assembly members:

Assembly members:
entity_1, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Data sets:
Data typeCount
13C chemical shifts324
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Musashi-1 C terminal deltaSeq1, msi1_ds21

Entities:

Entity 1, Musashi-1 C terminal deltaSeq1, msi1_ds2 127 residues - Formula weight is not available

1   METPROGLYTYRTHRTYRGLNPHEPROGLU
2   PHEARGVALGLUARGTHRPROLEUPROSER
3   ALAPROVALLEUPROGLULEUTHRALAILE
4   PROLEUTHRALATYRGLYPROMETALAALA
5   ALAALAALAALAALAALAVALVALARGGLY
6   THRGLYSERHISPROTRPTHRMETALAPRO
7   PROPROGLYSERTHRPROSERARGTHRGLY
8   GLYPHELEUGLYTHRTHRSERPROGLYPRO
9   METALAGLULEUTYRGLYALAALAASNGLN
10   ASPSERGLYVALSERSERTYRILESERALA
11   ALASERPROALAPROSERTHRGLYPHEGLY
12   HISSERLEUGLYGLYPROLEUILEALATHR
13   ALAPHETHRASNGLYTYRHIS

Samples:

sample_1: Musashi-1 C terminal domain delta-seq1, [U-98% 13C; U-98% 15N], 100 uM

sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks